(-)-Bicuculline methochloride

(HB0895)

Product overview

  • Name
    (-)-Bicuculline methochloride
  • Short description
    Competitive GABAA receptor antagonist
  • Biological description
    Methochloride salt of (+)-bicuculline. Competitive GABAA receptor antagonist. Also acts on calcium-dependent potassium channels.
  • Biological action
    Antagonist
  • Purity
    >99%
  • Our products in action

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Properties

  • Chemical name
    [R-(R*,S*)]-5-(6,8-Dihydro-8-oxofuro[3,4-e]-1,3-benzodioxol-6-yl)-5,6,7,8-tetrahydro- 6,6-dimethyl-1,3-dioxolo[4,5-g]isoquinolinium chloride
  • Molecular Weight
    417.85
  • Chemical structure
    (-)-Bicuculline methochloride  [53552-05-9]
  • Molecular Formula
    C21H20ClNO6
  • CAS Number
    53552-05-9
  • PubChem identifier
    44134574
  • SMILES
    C[N+]1(CCC2=CC3=C(C=C2C1C4C5=C(C6=C(C=C5)OCO6)C(=O)O4)OCO3)C.[Cl-]
  • Source
    Synthetic
  • InChi
    InChI=1S/C21H20NO6.ClH/c1-22(2)6-5-11-7-15-16(26-9-25-15)8-13(11)18(22)19-12-3-4-14-20(27-10-24-14)17(12)21(23)28-19;/h3-4,7-8,18-19H,5-6,9-10H2,1-2H3;1H/q+1;/p-1
  • InChiKey
    RLJKFAMYSYWMND-UHFFFAOYSA-M
  • MDL number
    MFCD00055233
  • Appearance
    Green solid

Applications

  • Application notes

    The GABAA receptor antagonist bicuculline is commonly used to reduce levels of inhibition by blocking the actions of the neurotransmitter GABA. It is commonly used at concentrations of 100 μM and above. Bicuculline methochloride from Hello Bio reduces both spontaneous inhibitory post synaptic currents (IPSC) and evoked IPSCs (see Fig 1 above). It was effective at 1 μM with complete receptor blockade at 100 μM.

     

    #Protocol 1: Evoked and spontaneous inhibitory post synaptic currents (IPSCs)

    • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
    • A stimulating electrode was placed in layers II/III and IPSCs were evoked by a single square (150 μs) pulse every 10 sec at a stimulus intensity that gave a reliable IPSC.
    • IPSCs were evoked at a range of neuron holding voltages to measure the reversal potential of the current to ensure it was GABAergic.
    • Neurons were held at 0mV and IPSCs continuously stimulated and recorded in response to 5 min applications of varying concentrations of Bicuculline methochloride until complete receptor inhibition.
    • Spontaneous IPSCs were recorded before and after addition of Bicuculline methochloride by holding the neuron at 0mV and recording for 10 sec.
    • All recordings for IPSCs were made in the presence of AMPAR antagonists.

Storing and Using Your Product

  • Storage instructions
    Room temperature
  • Solubility overview
    Soluble in water (100mM)

References for (-)-Bicuculline methochloride

  • Advantages of an antagonist: bicuculline and other GABA antagonists.

    Johnston GA (2013) Br J Pharmacol 169(2) : 328-36.
    PubMedID: 23425285
  • Differential effects of iontophoretic in vivo application of the GABA(A)-antagonists bicuculline and gabazine in sensory cortex.

    Kurt S et al (2006) Hear Res 212(1-2) : 224-35.
    PubMedID: 16442250
  • [Bicuculline inhibits airway remodeling in a murine model of chronic asthma].

    Zhu T et al (2010) Nan Fang Yi Ke Da Xue Xue Bao 30(4) : 842-6.
    PubMedID: 20423862

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