Fluorophore Labeling Calculator

The Hello Bio Fluorophore labeling calculator gives you an easy way to work out how much fluorescent dye you should add to your protein to ensure optimal labeling. This calculator is ideal for using with our antibody and protein labeling kits and is designed to be used with our Janelia Fluor® Conjugation Protocol.

Simply enter the mass and molecular weight of the protein alongside the dye to conjugate it with and the calculator will tell you the masses and volumes required for optimal labeling. We recommend starting with a Janelia Fluor®: Antibody molar ratio of 15.

Amount of each component

Janelia Fluor® (mass): -

Janelia Fluor® (volume): -

Antibody (volume): -

Buffer (volume): -

Other useful resources

If you need any further help with handling, storing or using your conjugated antibodies check our our technical FAQs for antibodies. We also have a wide range of extensively tested protocols available including for Western Blot (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC).

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When you get to the stage of planning your experiments, don't forget that we offer a range of agonists, antagonists, inhibitors, activators, antibodies and fluorescent tools that are really low cost (at up to half the price of other suppliers). You can use our Quick Multi-Search Tool to search for lots of products in one go, and the range includes:

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Life Scientist Guides and Scientist Support

You can find additional support and information on our blog, including Life Science Guides and support for scientists.

Need More Support?

Please reach out to our technical help team at technicalhelp@hellobio.com