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α-Galactosylceramide

(HB3751)
Data

Product overview

  • Name
    α-Galactosylceramide
  • Short description
    Immunostimulant. Natural killer T cell stimulator.
  • Biological description
    Synthetic. Originally isolated from the marine sponge Angelas mauritianus. Immunostimulant. Potent stimulator of natural killer T (NKT) cells. Specific ligand of the lipid-binding MHC class I-like protein CD1d in human and mouse NKT cells. Protects against LPS-induced shock. Potent antitumor compound.
  • Alternative names
    α-Gal-Cer; KRN7000
  • Purity
    >96%
  • Citations

Properties

  • Molecular Weight
    858.3
  • CAS Number
    158021-47-7
  • Source
    Originally isolated from the marine sponge Angelas mauritianus
  • InChiKey
    LSUQVAFARUJTHS-ABBGRRHVSA-N
  • Appearance
    Off-white solid

Storing and Using Your Product

  • Storage instructions
    -20°C
  • Solubility overview
    Solubilization in Tween20 or PBS for cell culture use. Also soluble in pyridine (not suitable for cell culture). Aliquot into glass vials.
  • Handling

    This compound is insoluble in water, methanol, ethanol and other organic solvents. It is very slightly soluble in tetrahydrofuran and pyridine. Some example protocols are as follows:


    Tween20 & Sodium Chloride, Heat/Sonicate

    • Dissolve α-GalCer in 0.5% Tween20, 0.9% NaCl solution
    • Heat to 85°C until solution turns cloudy
    • Take out of water bath und keep at RT
    • Solution will become clear quickly
    • If particles are left, sonicate (important: sonication has to be performed in glass vial, not in a plastic Eppendorf tube) and filter afterwards with 2 µm filter

    Tween20 & PBS, Heat/Sonicate

    • Dissolve the compound in 0.2mg/ml PBS containing 0.5% Tween20 (warm and sonicate for 2 hrs. at 37°C)
    • The solution can be more like a suspension than a clear solution
    • If particles are left, warm up and sonicate (important: sonication has to be performed in glass vial, not in a plastic Eppendorf tube)
    • For sonication no interval is recommended, just use a conventional water bath sonicator at standard amplitude

    DMSO, Heat/Sonicate

    • Dissolve the compound in DMSO at a concentration of 1mg/ml with heating at 80°C for several minutes
    • Sonication for 2 h
    • The solution of 1mg/ml in DMSO can be further diluted in PBS

  • Handling continued..

    2 Steps: Chloroform:Methanol > Evaporation > DMSO, PBS or Tween20 > Heat > Sonicate

    • Dissolve the compound in a mixture of chloroform:methanol (2:1)
    • Aliquot into reasonable aliquots for the experiment
    • Evaporate the solvent using a gentle stream of nitrogen so that you have a thin, dry film of material at the bottom of the vial
    • Add either DMSO or PBS + 0.5% Tween20 to achieve your final concentration
    • Some heating and sonication might be necessary
    • The thin film of compound generated by the evaporation step allows greater access of the compound to the solvent and better dissolution

    5.6% sucrose, 0.75% L-histidine and 0.5% Tween20 Heat Sonicate

    • Dissolve α-GalCer in 5.6% sucrose, 0.75% L-histidine and 0.5% Tween20
    • Heat at 80°C for several minutes and sonicate

  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for α-Galactosylceramide

  • Stimulation of invariant natural killer T cells by α-Galactosylceramide activates the JAK-STAT pathway in endothelial cells and reduces angiogenesis in the 5T33 multiple myeloma model.

    Nur et al (2014) Br J Haematol 167(5) : 651-63
    PubMedID: 25142285
  • α-Galactosylceramide stimulates splenic lymphocyte proliferation in vitro and increases antibody production in vivo in late neonatal-age mice.

    Chen and Ross (2015) Clin Exp Immunol 179(2) : 188-96
    PubMedID: 25178151
  • Activation of natural killer T cells by alpha-galactosylceramide in the presence of CD1d provides protection against colitis in mice.

    Saubermann et al (2000) Gastroenterology 119(1) : 119-28
    PubMedID: 10889161
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