Overview Aphidicolin is a potent DNA replication inhibitor which is often used to achieve cell synchronization.
Mechanism
Aphidicolin is a potent and specific inhibitor of B-family DNA polymerases and binds at or near the nucleotide-binding site. It prevents DNA polymerase-α from binding dNTPs without blocking the activity of DNA polymerase β or δ.
Aphidicolin inhibits DNA replication and some forms of DNA repair. During cell culture, addition of aphidicolin induces cell cycle pause at the G1/S border. DNA synthesis stops in cells that have entered S-phase, while nondividing cells are unaffected.
Uses Aphidicolin acts synergistically with vincristine and doxorubicin. In addition to its anti-mitotic effects, it exhibits antibiotic and antiviral activities.
Solubility & Handling
Storage instructions
+4°C
Solubility overview
Soluble in DMSO (25 mM)
Handling
This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the material in the dark and protecting from light.
Do not store the material in solution; make up solutions and use immediately.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
![(+)-Aphidicolin [38966-21-1] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb3690.png "(+)-Aphidicolin [38966-21-1]")
Understanding purity and quality - a guide for life scientists
