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Product overview

  • Name
  • Short description
    DNA replication inhibitor. Useful for cell synchronization
  • Biological description

    Aphidicolin is a potent DNA replication inhibitor which is often used to achieve cell synchronization.


    Aphidicolin is a potent and specific inhibitor of B-family DNA polymerases and binds at or near the nucleotide-binding site. It prevents DNA polymerase-α from binding dNTPs without blocking the activity of DNA polymerase β or δ.

    Aphidicolin inhibits DNA replication and some forms of DNA repair. During cell culture, addition of aphidicolin induces cell cycle pause at the G1/S border. DNA synthesis stops in cells that have entered S-phase, while nondividing cells are unaffected.

    Aphidicolin acts synergistically with vincristine and doxorubicin. In addition to its anti-mitotic effects, it exhibits antibiotic and antiviral activities.

  • Alternative names
    APC, APH, Aphidicoline, (+)-Aphidicolin, NSC234714, BRN4689958, ICI69653
  • Biological action
  • Purity
  • Citations


  • Chemical name
  • Molecular Weight
  • Chemical structure
    (+)-Aphidicolin [38966-21-1]
  • Molecular Formula
  • CAS Number
  • PubChem identifier
  • Source
    Isolated from Phoma sp. BS 7210
  • InChi
  • InChiKey
  • MDL number
  • Appearance
    White to off-white solid

Storing and Using Your Product

  • Storage instructions
  • Solubility overview
    Soluble in DMSO (25 mM)
  • Handling
    This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the material in the dark and protecting from light. Do not store the material in solution; make up solutions and use immediately.
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for Aphidicolin

  • Cell synchronization by inhibitors of DNA replication induces replication stress and DNA damage response: analysis by flow cytometry.

    Darzynkiewicz et al (2011) Methods Mol Biol. 761 : 85-96
    PubMedID: 21755443
  • Structural basis for inhibition of DNA replication by aphidicolin.

    Baranovskiy et al (2014) Nucleic Acids res. 42(22) : 14013-21
    PubMedID: 25429975
  • Aphidicolin inhibits the synthesis and joining of short DNA fragments but not the union of 10-kilobase DNA replication intermediates.

    Lonn et al (1983) Proc Natl Acad Sci U S A. 80(13) : 3996-9
    PubMedID: 6408640
  • Mechanism of DNA polymerase alpha inhibition by aphidicolin.

    Sheaff et al (1991) Biochemistry 30(35) : 8590-7
    PubMedID: 1909569
  • Inhibitor analysis of calf thymus DNA polymerases alpha, delta and epsilon.

    Wright et al (1994) FEBS lett. 341(1) : 128-30
    PubMedID: 8137912
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