BDNF (Brain-derived neurotrophic factor) is a member of the neurotrophin growth factor family and is highly expressed in the brain. BDNF activates the TrkB and p75 (P75^NTR) neurotrophin receptors.

BDNF plays an important role in brain development, physiology and pathology. Dysregulation of BDNF signaling is involved in several neurodegenerative disorders and BDNF also exerts effects on appetite, anxiety, cognition, reward and learning and memory.

Synaptic transmission and plasticity
BDNF is involved in the regulation of synaptic transmission and activity-dependent synaptic plasticity by both pre- and post- synaptic mechanisms. BDNF appears to strengthen excitatory (glutamtergic) synapses and weaken inhibitory (GABAergic) synapses.

BDNF contributes to adaptive neuronal responses such as LTP (long-term potentiation), LTD (long-term depression) and certain forms of short-term synaptic plasticity.

Neurogenesis
BDNF is an important regulator of neurogenesis and plays an important role in the developmental processes. BDNF has growth- and survival- promoting actions on various neurons, including dorsal root ganglion (DRG) cells and hippocampal and cortical neurons.

• Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
• Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days.
• For long term storage, a carrier protein (0.1% HSA or BSA) should be added to the stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.