BDNF (human)
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Product overview
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Name
BDNF (human)
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Short description
Member of the neurotrophin growth factor family. Plays an important role in synaptic plasticity and neurogenesis.
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Biological description
BDNF (Brain-derived neurotrophic factor) is a member of the neurotrophin growth factor family and is highly expressed in the brain. BDNF activates the TrkB and p75 (P75NTR) neurotrophin receptors.
BDNF plays an important role in brain development, physiology and pathology. Dysregulation of BDNF signaling is involved in several neurodegenerative disorders and BDNF also exerts effects on appetite, anxiety, cognition, reward and learning and memory.
Synaptic transmission and plasticity
BDNF is involved in the regulation of synaptic transmission and activity-dependent synaptic plasticity by both pre- and post- synaptic mechanisms. BDNF appears to strengthen excitatory (glutamtergic) synapses and weaken inhibitory (GABAergic) synapses.
BDNF contributes to adaptive neuronal responses such as LTP (long-term potentiation), LTD (long-term depression) and certain forms of short-term synaptic plasticity.
Neurogenesis
BDNF is an important regulator of neurogenesis and plays an important role in the developmental processes. BDNF has growth- and survival- promoting actions on various neurons, including dorsal root ganglion (DRG) cells and hippocampal and cortical neurons.
BDNF (human) is a recombinant protein expressed in and extracted from E. coli and purified to homogeneity. -
Species of origin
Human
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Alternative names
Brain-derived neurotrophic factor, MGC34632
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Biological action
Activator
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Purity
>97%
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Amino acids
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR.
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Citations
1 citation Submit Your Citation Now
Images
Properties
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Molecular Weight
27000
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CAS Number
218441-99-7
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Source
Escherichia Coli
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Appearance
White solid
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Formulation
Sterile filtered, white lyophilized powder
Applications
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Application notes
ED50 = 1.3-2 µg/ml (determined by the dose-dependent induction of C6 cells proliferation)
Storing and Using Your Product
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Storage instructions
-20°C
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Solubility overview
Soluble in sterile deionized water (not less than 100 µg/ml)
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Handling
- Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
- Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days.
- For long term storage, a carrier protein (0.1% HSA or BSA) should be added to the stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
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Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
References for BDNF (human)
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Brain-derived neurotrophic factor.
Binder and Scharfman (2004) Growth Factors 22(3) : 123-31PubMedID: 15518235 -
Cultured hippocampal neurons show responses to BDNF, NT-3, and NT-4, but not NGF.
Ip et al (1993) J Neurosci 13(8) : 3394-405PubMedID: 7688038 -
The ability of BDNF to modify neurogenesis and depressive-like behaviors is dependent upon phosphorylation of tyrosine residues 365/367 in the GABA(A)-receptor γ2 subunit.
Vithlani et al (2013) J Neurosci 33(39) : 15567-77PubMedID: 24068823 -
Neurotrophins: roles in neuronal development and function.
Huang and Reichardt (2001) Annu Rev Neurosci 24 : 677-736PubMedID: 11520916 -
BDNF function in adult synaptic plasticity: the synaptic consolidation hypothesis.
Bramham and Messaoudi (2005) Prog Neurobiol 76(2) : 99-125PubMedID: 16099088
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Increasing cellular lifespan with a flow system in organotypic culture of the Laterodorsal Tegmentum (LDT).
Romero-Leguizamón et al(2019) Sci Rep. 6(1) : 1486PubMedID: 30728375