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Brefeldin A (BFA)

(HB2949)
Data

Product overview

  • Name
    Brefeldin A (BFA)
  • Short description
    Reversible protein transport inhibitor
  • Biological description

    Brefeldin A is a reversible inhibitor of protein transport. 

    Following treatment with Brefeldin A, the Golgi complex disassembles and redistributes into the endoplasmic reticulum within minutes. Brefeldin A is a potent, rapid and reversible inhibitor of secretion.


    Brefeldin A inhibits the GTPase exchange factor acting on the ARF protein. ARF activates ADP-ribosylation factors to the golgi complex.


    Uses
    Brefeldin A is widely used in studies of membrane trafficking. It increases intracellular cytokine staining signals and is commonly used for intracellular staining of cytokines for flow cytometry. It blocks transport processes during cell activation and causes an accumulation of cytokines at the golgi complex/ endoplasmic reticulum.


    Brefeldin A also shows antibiotic actions and induces apoptosis and autophagy in mammalian cells. Recently, it has been shown to enhance CRISPR-mediated homology-directed repair (HDR) in hiPSCs (human induced pluripotent stem cells).


    Monensin sodium salt also available

  • Alternative names
    BFA, Synergisidin, Nectrolide, Decumbin, Cyanein
  • Biological action
    Inhibitor
  • Purity
    >98%
  • Citations

Properties

  • Chemical name
    1,6,7,8,9,11aβ,12,13,14,14αa-Decahydro-1β,13α-dihydroxy-6β-methyl-4H-cyclopent(f)oxacyclotridecin-4-one
  • Molecular Weight
    280.36
  • Chemical structure
    Brefeldin A BFA [20350-15-6]
  • Molecular Formula
    C16H24O4
  • CAS Number
    20350-15-6
  • PubChem identifier
    6436187
  • SMILES
    [H][C@]1(C)CCC\C=C\C2C[C@H](O)C[C@H]2[C@H](O)\C=C\C(=O)O1
  • InChi
    InChI=1S/C16H24O4/c1-11-5-3-2-4-6-12-9-13(17)10-14(12)15(18)7-8-16(19)20-11/h4,6-8,11-15,17-18H,2-3,5,9-10H2,1H3/b6-4+,8-7+/t11-,12?,13-,14+,15+/m0/s1
  • InChiKey
    KQNZDYYTLMIZCT-KFKPYADVSA-N
  • MDL number
    MFCD12913297
  • Appearance
    White to off-white solid

Storing and Using Your Product

  • Storage instructions
    -20°C (desiccate)
  • Solubility overview
    Soluble in DMSO (50mM) and in ethanol (10mM)
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

References for Brefeldin A (BFA)

  • Brefeldin A: the advantage of being uncompetitive.

    Chardin and McCormick (1999) Cell 97(2) : 153-5
    PubMedID: 10219235
  • Golgi tubule traffic and the effects of brefeldin A visualized in living cells.

    Sciaky et al (1997) J Cell Biol 39(5) : 1137-55
    PubMedID: 9382862
  • Small molecules enhance CRISPR genome editing in pluripotent stem cells.

    Yu et al (2015) Cell Stem Cell 16(2) : 142-7
    PubMedID: 25658371
  • Detection of intracellular cytokines by flow cytometry.

    Jung et al (1993) J Immunol Methods. 159(1-2) : 197-207
    PubMedID: 8445253
  • Molecular mechanism and functional role of brefeldin A-mediated ADP-ribosylation of CtBP1/BARS.

    Colanzi et al (2013) Proc Natl Acad Sci U S A 110(24) : 9794-9
    PubMedID: 23716697
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