Brefeldin A (BFA)
Reversible protein transport inhibitor
Brefeldin A is a reversible inhibitor of protein transport.
Following treatment with Brefeldin A, the Golgi complex disassembles and redistributes into the endoplasmic reticulum within minutes. Brefeldin A is a potent, rapid and reversible inhibitor of secretion.
Brefeldin A inhibits the GTPase exchange factor acting on the ARF protein. ARF activates ADP-ribosylation factors to the golgi complex.
Brefeldin A is widely used in studies of membrane trafficking. It increases intracellular cytokine staining signals and is commonly used for intracellular staining of cytokines for flow cytometry. It blocks transport processes during cell activation and causes an accumulation of cytokines at the golgi complex/ endoplasmic reticulum.
Brefeldin A also shows antibiotic actions and induces apoptosis and autophagy in mammalian cells. Recently, it has been shown to enhance CRISPR-mediated homology-directed repair (HDR) in hiPSCs (human induced pluripotent stem cells).
Monensin sodium salt also available
BFA, Synergisidin, Nectrolide, Decumbin, Cyanein
White to off-white solid
Storing and Using Your Product
Soluble in DMSO (50mM) and in ethanol (10mM)
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
References for Brefeldin A (BFA)
Brefeldin A: the advantage of being uncompetitive.Chardin and McCormick (1999) Cell 97(2) : 153-5PubMedID: 10219235
Golgi tubule traffic and the effects of brefeldin A visualized in living cells.Sciaky et al (1997) J Cell Biol 39(5) : 1137-55PubMedID: 9382862
Small molecules enhance CRISPR genome editing in pluripotent stem cells.Yu et al (2015) Cell Stem Cell 16(2) : 142-7PubMedID: 25658371
Detection of intracellular cytokines by flow cytometry.Jung et al (1993) J Immunol Methods. 159(1-2) : 197-207PubMedID: 8445253
Molecular mechanism and functional role of brefeldin A-mediated ADP-ribosylation of CtBP1/BARS.Colanzi et al (2013) Proc Natl Acad Sci U S A 110(24) : 9794-9PubMedID: 23716697