Product overview

  • Name
    Calcein-AM
  • Short description
    Non- fluorescent, cell permeable dye
  • Biological description
    Non- fluorescent and cell permeable dye. Hydrolysed into green fluorescent calcein by cytosolic esterases in living cells. Uses include monitoring chemotaxis, multidrug resistance and determining cell viability.
  • Biological action
    Dyes & stains
  • Purity
    >95%
  • Our products in action

Properties

  • Chemical name
    CAL-AM
  • Molecular Weight
    994.86
  • Chemical structure
    Calcein-AM  [148504-34-1]
  • Molecular Formula
    C46H46N2O23
  • CAS Number
    148504-34-1
  • PubChem identifier
    http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=390986
  • SMILES
    CC(=O)OCOC(=O)CN(CC1=C(C=CC2=C1OC3=C(C24C5=CC=CC=C5C(=O)O4)C=CC(=C3CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OC(=O)C)OC(=O)C)CC(=O)OCOC(=O)C

Storing and Using Your Product

  • Storage instructions
    -20°C
  • Solubility overview
    soluble in DMSO (10mg/ml) or water
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for Calcein-AM

  • Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging.

    Bratosin D et al (2005) Cytometry A 66(1) : 78-84.
    PubMedID: 15915509
  • The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay.

    De Gendt CM et al (1996) Clin Chim Acta 249(1-2) : 189-95.
    PubMedID: 8737602
  • Membrane topology and glycosylation of the human multidrug resistance-associated protein.

    Bakos E et al (1996) J Biol Chem 271(21) : 12322-6.
    PubMedID: 8647833

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