CA200623 CellAura fluorescent adenosine agonist [NECA]

(HB7813)

Product overview

  • Name
    CA200623 CellAura fluorescent adenosine agonist [NECA]
  • Short description
    Fluorescent adenosine receptor agonist
  • Biological description
    Fluorescent adenosine receptor agonist (apparent KD values are 8.57, 8.47, 6.76 and 5.69 for A3, A1, A2A and A2B respectively). Also inhibits forskolin-stimulated cAMP accumulation (pIC50 = 8.57) and [3H]-inositol phosphate accumulation (pEC50 = 7.34). A fluorescent adenosine receptor ligand derived from NECA, non-selective adenosine agonist.
  • Alternative names
    Fluorescent Adenosine receptor Agonist (A-633-AG), A-633-AG, ABEA-X-BY630
  • Biological action
    Agonist
  • Purity
    >97%
  • Citations

Images

Properties

  • Molecular Weight
    925
  • Source
    Synthetic
  • Appearance
    Purple solid
  • Formulation
    Lyophilized film
  • Excitation
    638 nm
  • Emission
    657 nm

Applications

  • Application notes
    For ligand binding; fluorescence imaging; high content analysis; kinetic analysis; cell sorting at adenosine A1 / A2A / A3 receptors use solutions up to 100 nM.
  • Pharmacological validation
    For full experimental data see: J. Med. Chem. 2007, 50, 782-793; FASEB J. 2008, 22, 850-860

Storing and Using Your Product

  • Storage instructions
    -20°C, protect from light
  • Solubility overview
    Soluble in DMSO
  • Handling
    After thawing individual aliquots for use, we recommend briefly sonicating the sample to ensure it is fully dissolved and the solution is homogeneous. We do not recommend using the product after subjecting it to repetitive freeze-thaw cycles.
  • Shipping conditions
    The product, supplied in a dry form, is stable at ambient temperature for periods of up to a few days and does not require shipping on ice/dry ice.
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
The following papers have cited the use of CA200623 CellAura fluorescent adenosine agonist [NECA] (HB7813) from Hello Bio. If you have published a paper using this product, and it is not shown here, then please tell us! We will send you a free gift as a thank you!
  • Influence of fluorophore and linker composition on the pharmacology of fluorescent adenosine A1 receptor ligands.

    Baker JG et al. (2010) Br J Pharmacol 159(4) : 772-86.
    PubMedID: 20105183
  • Pharmacology under the microscope: the use of fluorescence correlation spectroscopy to determine the properties of ligand-receptor complexes.

    Briddon SJ et al. (2007) Trends Pharmacol Sci 28(12) : 637-45.
    PubMedID: 18001848
  • Agonist-occupied A3 adenosine receptors exist within heterogeneous complexes in membrane microdomains of individual living cells.

    Cordeaux Y et al. (2008) FASEB J 22(3) : 850-60.
    PubMedID: 17959910
  • Adenosine receptors and membrane microdomains.

    Lasley RD(2011) Biochim Biophys Acta 1808(5) : 1284-9.
    PubMedID: 20888790
  • Metabotropic purinergic receptors in lipid membrane microdomains.

    D' Ambrosi N et al. (2013) Curr Med Chem 20(1) : 56-63.
    PubMedID: 23151003
  • Developments in fluorescent probes for receptor research.

    Leopoldo M et al. (2009) Drug Discov Today 14(13-14) : 706-12.
    PubMedID: 19573791