CA200773 CellAura fluorescent D1 antagonist [SKF83566-green]

(HB7822)

Product overview

  • Name
    CA200773 CellAura fluorescent D1 antagonist [SKF83566-green]
  • Short description
    Fluorescent D1 dopamine receptor antagonist
  • Biological description
    Selective fluorescent D1 dopamine receptor antagonist (apparent KD values are 7.09, < 5 and 7.56 for D1, D2 and D5 receptors respectively). Also antagonizes the activity of SKF 83566, a D1 dopamine receptor agonist. Displays no intrinsic agonist activity.
  • Alternative names
    CA200773
  • Biological action
    Antagonist
  • Purity
    >97%
  • Our products in action

Images

Properties

  • Molecular Weight
    877
  • Source
    Synthetic
  • Formulation
    Lyophilized film
  • Excitation
    488 nm
  • Emission
    525 / 550 nm

Applications

  • Application notes
    For imaging at the D1 receptor use solutions up to 100 nM.
  • Pharmacological validation
    The CellAura fluorescent D1 antagonist [SKF83566-green] ligand was shown to antagonize the activity of the D1 agonist, SKF 83566, in a recombinant CHO cell line expressing the human D1 receptor and a cyclic AMP-responsive secreted placental alkaline phosphatase (SPAP) reporter gene. The cyclic AMP-induced expression of SPAP was measured under basal and forskolin-stimulated (maximal) conditions. Addition of CellAura fluorescent D1 antagonist [SKF83566-green] to the basal or forskolin-stimulated cells did not significantly alter basal and stimulated SPAP levels, demonstrating that CellAura fluorescent D1 antagonist [SKF83566-green] has no intrinsic agonist activity. To determine the apparent KD for CellAura fluorescent D1 antagonist [SKF83566-green], cells were treated with varying concentrations of SKF 83566 alone, or in the presence of 1µM CellAura fluorescent D1 antagonist [SKF83566-green], and the cyclic AMP-induced expression of SPAP measured. The apparent KD was calculated from the rightward shift of the agonist response curve in the presence of CellAura fluorescent D1 antagonist [SKF83566-green], compared to the response curve for the agonist alone.

Storing and Using Your Product

  • Storage instructions
    -20°C, protect from light
  • Solubility overview
    Soluble in DMSO
  • Handling
    After thawing individual aliquots for use, we recommend briefly sonicating the sample to ensure it is fully dissolved and the solution is homogeneous. We do not recommend using the product after subjecting it to repetitive freeze-thaw cycles.
  • Shipping conditions
    The product, supplied in a dry form, is stable at ambient temperature for periods of up to a few days and does not require shipping on ice/dry ice.
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
The following papers have cited the use of CA200773 CellAura fluorescent D1 antagonist [SKF83566-green] (HB7822) from Hello Bio. If you have published a paper using this product, and it is not shown here, then please tell us! We will send you a free gift as a thank you!
  • High Throughput Fluorescence-Based Live Cell Binding Assays For G Protein-Coupled Receptors

    Arruda et al. () Proceedings of the British Pharmacological Society :