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CHIR 99021 trihydrochloride

(HB1262)
Data

Product overview

  • Name
    CHIR 99021 trihydrochloride
  • Short description
    Potent, selective GSK3 inhibitor. Wnt signaling activator. Water soluble.
  • Biological description

    Potent, selective and ATP-competitive GSK3 β inhibitor (IC50 values are 6.7 and 10 nM for GSK-3β and GSK-3α respectively).


    Water soluble hydrochloride salt of CHIR 99021.


    Wnt signaling activator which is commonly used with PD 032501 as part of the 2i condition.


    Exhibits no cross reactivity against CDKs and exhibits >500-fold selectivity for GSK3 over other protein kinases and >800-fold selectivity over 20+ other enzymes and receptors.


    Promotes self-renewal of embryonic stem cells and enables mouse embryonic fibroblast (MEF) reprogramming into iPSCs.

  • Biological action
    Inhibitor
  • Purity
    >98%
  • Citations

Properties

  • Chemical name
    6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethy l]amino]-3-pyridinecarbonitrile trihydrochloride
  • Molecular Weight
    574.72
  • Chemical structure
    CHIR 99021 trihydrochloride [1782235-14-6]
  • Molecular Formula
    C22H18Cl2N8.3HCl
  • CAS Number
    1782235-14-6
  • PubChem identifier
    78243722
  • SMILES
    CC1=CN=C(N1)C2=CN=C(N=C2C3=C(C=C(C=C3)Cl)Cl)NCCNC4=NC=C(C=C4)C#N.Cl.Cl.Cl
  • Source
    Synthetic
  • InChi
    InChI=1S/C22H18Cl2N8.3ClH/c1-13-10-29-21(31-13)17-12-30-22(32-20(17)16-4-3-15(23)8-18(16)24)27-7-6-26-19-5-2-14(9-25)11-28-19;;;/h2-5,8,10-12H,6-7H2,1H3,(H,26,28)(H,29,31)(H,27,30,32);3*1H
  • InChiKey
    DSFVSCNMMZRCIA-UHFFFAOYSA-N
  • MDL number
    MFCD11846251

Storing and Using Your Product

  • Storage instructions
    -20°C
  • Solubility overview
    Soluble in water (25mM) and in DMSO (50mM)
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for CHIR 99021 trihydrochloride

  • The roles of Notch3 on the cell proliferation and apoptosis induced by CHIR99021 in NSCLC cell lines: a functional link between Wnt and Notch signaling pathways.

    Li C et al (2013) PLoS One 8(12) : e84659.
    PubMedID: 24367688
  • Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2.

    Li W et al (2009) Stem cells 27(12) : 2992-3000
    PubMedID: 19839055
  • Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains.

    Ye S et al (2012) PLoS One 7(4) : e35892.
    PubMedID: 22540008
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