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Product overview

  • Name
    CHIR 99021
  • Short description
    Potent, selective GSK3 inhibitor. Wnt signaling activator.
  • Biological description

    Potent, selective and ATP-competitive GSK-3 inhibitor (IC50 values are 6.7 and 10 nM for GSK-3β and GSK-3α respectively).

    Wnt signaling activator which is commonly used with PD 032501 as part of the 2i condition.

    Exhibits no cross reactivity against CDKs and exhibits >500-fold selectivity for GSK3 over other protein kinases and >800-fold selectivity over >20 other enzymes and receptors.

    Promotes self-renewal of embryonic stem cells and enables mouse embryonic fibroblast (MEF) reprogramming into iPSCs.

    Water soluble CHIR 99021 trihydrochloride also available.

  • Alternative names
  • Biological action
  • Purity
  • Citations


  • Chemical name
    6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5 -methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile
  • Molecular Weight
  • Chemical structure
    CHIR 99021  [252917-06-9]
  • Molecular Formula
  • CAS Number
  • PubChem identifier
  • InChi
  • InChiKey
  • MDL number

Storing and Using Your Product

  • Storage instructions
  • Solubility overview
    Soluble in DMSO (20mM)
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for CHIR 99021

  • The roles of Notch3 on the cell proliferation and apoptosis induced by CHIR99021 in NSCLC cell lines: a functional link between Wnt and Notch signaling pathways.

    Li C et al (2013) PLoS One 8(12) : e84659.
    PubMedID: 24367688
  • Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2.

    Li W et al (2009) Stem cells 27(12) : 2992-3000
    PubMedID: 19839055
  • Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains.

    Ye S et al (2012) PLoS One 7(4) : e35892.
    PubMedID: 22540008
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