CHIR 99021

(HB1261)

Product overview

  • Name
    CHIR 99021
  • Short description
    Potent, selective GSK3 inhibitor. Wnt signaling activator.
  • Biological description

    Potent, selective and ATP-competitive GSK-3 inhibitor (IC50 values are 6.7 and 10 nM for GSK-3β and GSK-3α respectively).


    Wnt signaling activator which is commonly used with PD 032501 as part of the 2i condition.


    Exhibits no cross reactivity against CDKs and exhibits >500-fold selectivity for GSK3 over other protein kinases and >800-fold selectivity over >20 other enzymes and receptors.


    Promotes self-renewal of embryonic stem cells and enables mouse embryonic fibroblast (MEF) reprogramming into iPSCs.


    Water soluble CHIR 99021 trihydrochloride also available.

  • Alternative names
    CHIR99021
  • Biological action
    Inhibitor
  • Purity
    >98%
  • Our products in action

Properties

  • Chemical name
    6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5 -methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile
  • Molecular Weight
    465.34
  • Chemical structure
    CHIR 99021  [252917-06-9]
  • Molecular Formula
    C22H18Cl2N8
  • CAS Number
    252917-06-9
  • PubChem identifier
    9956119
  • SMILES
    CC1=CN=C(N1)C2=CN=C(N=C2C3=C(C=C(C=C3)Cl)Cl)NCCNC4=NC=C(C=C4)C#N
  • InChi
    InChI=1S/C22H18Cl2N8/c1-13-10-29-21(31-13)17-12-30-22(32-20(17)16-4-3-15(23)8-18(16)24)27-7-6-26-19-5-2-14(9-25)11-28-19/h2-5,8,10-12H,6-7H2,1H3,(H,26,28)(H,29,31)(H,27,30,32)
  • InChiKey
    AQGNHMOJWBZFQQ-UHFFFAOYSA-N
  • MDL number
    MFCD11846251

Storing and Using Your Product

  • Storage instructions
    -20°C
  • Solubility overview
    Soluble in DMSO (20mM)
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for CHIR 99021

  • The roles of Notch3 on the cell proliferation and apoptosis induced by CHIR99021 in NSCLC cell lines: a functional link between Wnt and Notch signaling pathways.

    Li C et al (2013) PLoS One 8(12) : e84659.
    PubMedID: 24367688
  • Selective glycogen synthase kinase 3 inhibitors potentiate insulin activation of glucose transport and utilization in vitro and in vivo.

    Ring DB et al (2003) Diabetes 52(3) : 588-95.
    PubMedID: 12606497
  • Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains.

    Ye S et al (2012) PLoS One 7(4) : e35892.
    PubMedID: 22540008