Crystal Violet

(HB0742)

Product overview

  • Name
    Crystal Violet
  • Short description
    Cation dye used in Gram staining
  • Alternative names
    Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B
  • Biological description
    Cation dye. Used in Gram staining to distinguish between gram positive and negative bacteria. Interacts with aqueous form of Kl-I2 during gram staining producing a chemical precipitate. Displays colour changes at different pHs (pH0.0- yellow, pH 2.0- blue/violet). Also used to measure Cytostatic/cytotoxic effects on tumour cell lines.
  • Biological action
    Dyes & stains
  • Citations

Properties

  • Chemical name
    Gentian Violet; Hexamethylpararosaniline chloride
  • Molecular Weight
    407.99
  • Chemical structure
    Crystal Violet  [548-62-9]
  • Molecular Formula
    C25H30N3Cl
  • CAS Number
    548-62-9
  • PubChem identifier
    http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=11057
  • SMILES
    [Cl-].CN(C)C1=CC=C(C=C1)C(\C1=CC=C(C=C1)N(C)C)=C1/C=C\C(\C=C/1)=[N+](/C)C

Storing and Using Your Product

  • Storage instructions
    room temperature
  • Solubility overview
    soluble in water (50mg/ml, at 27 oC) or chloroform
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for Crystal Violet

  • Statins potentiate cytostatic/cytotoxic activity of sorafenib but not sunitinib against tumor cell lines in vitro.

    Bil J et al (2010) Cancer Lett 288(1) : 57-67.
    PubMedID: 19632769
  • Chemical mechanism of the Gram stain and synthesis of a new electron-opaque marker for electron microscopy which replaces the iodine mordant of the stain.

    Davies JA et al (1983) J Bacteriol 156(2) : 837-45.
    PubMedID: 6195147
  • Crystal violet as an i-motif structure probe for reversible and label-free pH-driven electrochemical switch.

    Zhang XY et al (2014) Anal Biochem 455 : 55-9.
    PubMedID: 24699211

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