γ-secretase inhibitor. Classical notch inhibitor. Induces neuronal differentiation
DAPT is a γ-secretase inhibitor which blocks notch signaling. It is a classical notch inhibitor.
It has been reported to inhibit amyloid beta (Aβ) in a dose-dependent manner although at low doses, an increase in Aβ production in cell culture and animal models has also been reported.
Uses & applications
DAPT is used in a variety of stem cell applications.
It has been shown that in combination with other small molecules that inhibit SMAD (SB 431542, LDN193189), activate WNT (CHIR99021) and inhibit FGF-signaling (SU5402), DAPT yields 75% post-mitotic neurons in 11 days of differentiation. These are defined as peripheral sensory neurons.
Recently, DAPT in combination with LDN193189, SB431542, XAV939, PD0325901 and SU5402 has been shown to accelerate induction of early-born cortical neurons that have mature electrophysiological properties by day 16 of differentiation.
DAPT also enhances neuronal differentiation in ESC-derived embryoid bodies independent of Hh-signaling.
Additionally, the compound promotes cardiac differentiation of murine pluripotent stem cells and enhances the reprogramming of mouse fibroblasts to iCLMs (induced cardiac-like myocytes).
N-[N-(3,5-Difluorophenacetyl-L-alanyl)]-S-phenylglycine tbutyl ester
White to off-white solid
Storing and Using Your Product
Soluble in DMSO (100mM)
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
References for DAPT
The Notch signaling inhibitor DAPT down-regulates cdk5 activity and modulates the distribution of neuronal cytoskeletal proteins.Kanungo et al (2008) J Neurochem 106(5) : 2236-48PubMedID: 18662245
The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived embryoid bodies independently of sonic hedgehog signaling.Crawford and Roelink (2007) Dev Dyn 236(3) : 886-92PubMedID: 17295317
Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brain.Dovey et al (2001) J Neurochem 76(1) : 173-81PubMedID: 11145990
Lingo-1 shRNA and Notch signaling inhibitor DAPT promote differentiation of neural stem/progenitor cells into neurons.Wang et al (2015) Brain Res 8993(15) : 873-2PubMedID: 26607252