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Data

Product overview

  • Name
    DAPT
  • Short description
    γ-secretase inhibitor. Classical notch inhibitor. Induces neuronal differentiation
  • Biological description

    Overview

    DAPT is a γ-secretase inhibitor which blocks notch signaling. It is a classical notch inhibitor.

    It has been reported to inhibit amyloid beta (Aβ) in a dose-dependent manner although at low doses, an increase in Aβ production in cell culture and animal models has also been reported.

     

    Uses & applications

    DAPT is used in a variety of stem cell applications.

    It has been shown that in combination with other small molecules that inhibit SMAD (SB 431542, LDN193189), activate WNT (CHIR99021) and inhibit FGF-signaling (SU5402), DAPT yields 75% post-mitotic neurons in 11 days of differentiation. These are defined as peripheral sensory neurons.

    Recently, DAPT in combination with LDN193189, SB431542, XAV939, PD0325901 and SU5402 has been shown to accelerate induction of early-born cortical neurons that have mature electrophysiological properties by day 16 of differentiation.

    DAPT also enhances neuronal differentiation in ESC-derived embryoid bodies independent of Hh-signaling.

    Additionally, the compound promotes cardiac differentiation of murine pluripotent stem cells and enhances the reprogramming of mouse fibroblasts to iCLMs (induced cardiac-like myocytes).

     

  • Biological action
    Inhibitor
  • Purity
    >98%
  • Citations

Properties

  • Chemical name
    N-[N-(3,5-Difluorophenacetyl-L-alanyl)]-S-phenylglycine tbutyl ester
  • Molecular Weight
    432.5
  • Chemical structure
    DAPT [208255-80-5]
  • Molecular Formula
    C23H26F2N2O4
  • CAS Number
    208255-80-5
  • PubChem identifier
    5311272
  • SMILES
    C[C@@H](C(=O)N[C@@H](C1=CC=CC=C1)C(=O)OC(C)(C)C)NC(=O)CC2=CC(=CC(=C2)F)F
  • Source
    Synthetic
  • InChi
    InChI=1S/C23H26F2N2O4/c1-14(26-19(28)12-15-10-17(24)13-18(25)11-15)21(29)27-20(16-8-6-5-7-9-16)22(30)31-23(2,3)4/h5-11,13-14,20H,12H2,1-4H3,(H,26,28)(H,27,29)/t14-,20-/m0/s1
  • InChiKey
    DWJXYEABWRJFSP-XOBRGWDASA-N
  • MDL number
    MFCD04974585
  • Appearance
    White to off-white solid

Storing and Using Your Product

  • Storage instructions
    +4°C
  • Solubility overview
    Soluble in DMSO (100mM)
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for DAPT

  • The Notch signaling inhibitor DAPT down-regulates cdk5 activity and modulates the distribution of neuronal cytoskeletal proteins.

    Kanungo et al (2008) J Neurochem 106(5) : 2236-48
    PubMedID: 18662245
  • The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived embryoid bodies independently of sonic hedgehog signaling.

    Crawford and Roelink (2007) Dev Dyn 236(3) : 886-92
    PubMedID: 17295317
  • Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brain.

    Dovey et al (2001) J Neurochem 76(1) : 173-81
    PubMedID: 11145990
  • Lingo-1 shRNA and Notch signaling inhibitor DAPT promote differentiation of neural stem/progenitor cells into neurons.

    Wang et al (2015) Brain Res 8993(15) : 873-2
    PubMedID: 26607252
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