The Hello Bio Dilution Calculator gives you an easy way to work out how to dilute stock solutions of known concentrations.
There are 4 parameters: concentration (C1) and volume (V1) of the stock solution and the concentration (C2) and volume (V2) of the desired working solution. Just input any 3 of the 4 parameters – and the calculator will work out the fourth!
How does it work? The molarity equation
The Hello Bio molarity calculator uses the following equation:
Concentration1 x Volume1 = Concentration2 x Volume2
This can be abbreviated to C1V1 = C2V2
How to use the calculator - a worked example
Below is an example calculation showing what volume of a 10mM stock solution is required to make 50mL of a 100μL working solution.
We will use the equation C1V1=C2V2 where C1=10mM, C2=100μM, V2=50mL and V1 is the unknown
1. Enter 10 into the 'Concentration 1' box and select millimolar as a unit
2. Enter 100 into the 'Concentration 2' box and select micromolar as a unit
3. Enter 50 into the 'Volume 2' box and select milliliter as a unit
4. Press calculate
5. The answer of 0.5 milliliter (mL) appears in the 'Volume 1' box