DL-AP5 sodium salt

(HB0252)

Product overview

  • Name
    DL-AP5 sodium salt
  • Short description
    Competitive NMDA receptor antagonist. Sodium salt.
  • Biological description

    Competitive NMDA receptor antagonist. Sodium salt. Binds at the glutamate site. Impairs learning and fear conditioning. DL-AP5 also available.

  • Alternative names
    DL-APV sodium salt
  • Biological action
    Antagonist
  • Purity
    >98%
  • Our products in action

Images

Properties

  • Chemical name
    DL-2-Amino-5-phosphonopentanoic acid sodium salt
  • Molecular Weight
    219.11
  • Chemical structure
    DL-AP5 sodium salt  [1303993-72-7]
  • Molecular Formula
    C5H11NNaO5P
  • CAS Number
    1303993-72-7
  • PubChem identifier
    52974251
  • SMILES
    C(CC(C(=O)O)N)CP(=O)(O)[O-].[Na+]
  • Source
    Synthetic
  • InChi
    InChI=1S/C5H12NO5P.Na/c6-4(5(7)8)2-1-3-12(9,10)11;/h4H,1-3,6H2,(H,7,8)(H2,9,10,11);/q;+1/p-1
  • InChiKey
    KWRCYAPNGUCHOE-UHFFFAOYSA-M
  • Appearance
    White solid

Applications

  • Application notes

    DL-AP5 sodium salt is a water soluble NMDA receptor antagonist. It is typically used at a concentration of 50–100 µM. DL-AP5 sodium salt from Hello Bio reduces evoked NMDAR current with full receptor antagonism at 50 µM (see Fig 1 above), consistent with the literature for this compound.

     

    #Protocol 1: Evoked NMDAR currents at +40 mV

    • NMDAR currents were recorded via whole cell voltage clamp recordings of CA1 pyramidal neurons from rat hippocampal brain slice.
    • NMDAR currents were evoked via a stimulating electrode placed in the CA3 region to stimulate the Schaffer collateral pathway.
    • Each NMDAR current was evoked via a single square (150 µs) pulse every 10 sec at a stimulus intensity that gave a reliable NMDAR current.
    • Neurons were constantly held at +40 mV and NMDAR currents recorded in response to continual bath applications of NMDAR antagonists.
    • All NMDAR recordings were made in the presence of GABAA-R and AMPAR antagonists.

Storing and Using Your Product

  • Storage instructions
    Room temperature (desiccate)
  • Solubility overview
    Soluble in water (100mM)
  • Handling
    Hydroscopic solid, contact with air may cause material to become sticky. Product performance should not be affected but we recommend storing the material in a sealed jar.
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for DL-AP5 sodium salt

  • Infusion of the NMDA receptor antagonist, DL-APV, into the basolateral amygdala disrupts learning to fear a novel and a familiar context as well as relearning to fear an extinguished context.

    Laurent V et al (2009) Learn Mem 16(1) : 96-105.
    PubMedID: 19141468
  • The basolateral amygdala is necessary for learning but not relearning extinction of context conditioned fear.

    Laurent V et al (2008) Learn Mem 15(5) : 304-14.
    PubMedID: 18463174
  • Comparative analysis of different competitive antagonists interaction with NR2A and NR2B subunits of NMDA ionotropic glutamate receptor.

    Blaise MC et al (2005) J Mol Model 11(6) : 489-502.
    PubMedID: 15928921