Fluorescein Isothiocyanate (FITC) labeled Phalloidin binds and labels F-actin but not G-actin.
It is a green fluorescent stain which allows high-contrast discrimination of actin. Non-specific binding of phalloidin is negligible and it provides more intense labeling of F-actin than antibodies.
Uses and applications
FITC Phalloidin has a wide range of applications and can be used with formaldehyde-fixed and permeabilized tissue sections, cell cultures and cell-free experiments samples. It may also be used with de-paraffinized paraffin-embedded samples.
Purity
>93%
Description
Green fluorescent cytoskeleton stain. Binds and labels F-actin.
Figure 1. FITC Phalloidin and DAPI co-staining in a fibroblast observed in neuronal cell culture
FITC Phalloidin shows high affinity for actin therefore is used to label actin filaments in immunofluorescence experiments. FITC Phalloidin from Hello Bio labels actin filaments (green) at 183nM when counterstained with DAPI (blue — HBO747, 1 µg/ml). For protocol see #Protocol 1 in application notes below.
Figure 2. FITC Phalloidin, GFAP and DAPI co-staining in neuronal cell culture
FITC Phalloidin shows high affinity for actin therefore is used to label actin filaments in immunofluorescence experiments. FITC Phalloidin from Hello Bio labels actin filaments (green) at 183nM when co-stained with an anti-GFAP antibody (red) and DAPI (blue — HBO0747, 1 µg/ml). For protocol see #Protocol 1 in application notes below.
Figure 3. FITC Phalloidin, GFAP and DAPI co- staining in neuronal cell culture
FITC Phalloidin shows high affinity for actin therefore is used to label actin filaments in immunofluorescence experiments. FITC Phalloidin from Hello Bio labels actin filaments (green) at 183 nM when co-stained with an anti-GFAP antibody (red) and DAPI (blue — HB0747, µg/ml). For protocol see #Protocol 1 in application notes below.
FITC Phalloidin product vial image | Hello Bio
Figure 1. FITC Phalloidin and DAPI co-staining in a fibroblast observed in neuronal cell culture
FITC Phalloidin shows high affinity for actin therefore is used to label actin filaments in immunofluorescence experiments. FITC Phalloidin from Hello Bio labels actin filaments (green) at 183nM when counterstained with DAPI (blue — HBO747, 1 µg/ml). For protocol see #Protocol 1 in application notes below.
Figure 2. FITC Phalloidin, GFAP and DAPI co-staining in neuronal cell culture
FITC Phalloidin shows high affinity for actin therefore is used to label actin filaments in immunofluorescence experiments. FITC Phalloidin from Hello Bio labels actin filaments (green) at 183nM when co-stained with an anti-GFAP antibody (red) and DAPI (blue — HBO0747, 1 µg/ml). For protocol see #Protocol 1 in application notes below.
Figure 3. FITC Phalloidin, GFAP and DAPI co- staining in neuronal cell culture
FITC Phalloidin shows high affinity for actin therefore is used to label actin filaments in immunofluorescence experiments. FITC Phalloidin from Hello Bio labels actin filaments (green) at 183 nM when co-stained with an anti-GFAP antibody (red) and DAPI (blue — HB0747, µg/ml). For protocol see #Protocol 1 in application notes below.
FITC Phalloidin product vial image | Hello Bio
Biological Data
Application notes
For our 300 tests pack, to make your stock solution, you should dissolve the contents of the vial in 1.5 mL of methanol or DMSO.
#Protocol 1: FITC-Phalloidin labelling of primary cultured neurones.
Primary neurones were isolated and cultured from P2 rats and grown for three weeks before being fixed with 4% paraformaldehyde.
Coverslips containing neuronal cell cultures were labelled for GFAP following standard immunohistochemical approaches.
The secondary antibody was incubated for 1 hour with the addition of FITC Phalloidin (183nM, 1:40 dilution of staining solution)
Coverslips were then submerged in 1µg/ml DAPI diluted in PBS for 1 minute.
Coverslips were mounted and imaged with a fluorescent microscope.
Solubility & Handling
Storage instructions
-20°C
Solubility overview
Soluble in DMSO (NB: may appear colourless in very dry solvent)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
![Phalloidin, Fluorescein Isothiocyanate Labeled | [] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb0814_1.png "Phalloidin, Fluorescein Isothiocyanate Labeled | []")
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