Fura-2 AM

(HB0780)

Product overview

  • Name
    Fura-2 AM
  • Short description
    High affinity, cell permeable calcium indicator which is ratiometric and UV light excitable
  • Biological description

    High affinity, cell permeable calcium indicator which is ratiometric and UV light excitable. AM ester derivative of Fura-2. Can noninvasively be loaded into live cells by incubation. Widely used for ratio-imaging microscopy and measures intracellular calcium elevations in neurons and other excitable cells. Excitation 340/380nm, Emission 505nm.

  • Biological action
    Dyes & stains
  • Purity
    >95%
  • Citations

Properties

  • Chemical name
    1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-amino-5'-methyl-phenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester
  • Molecular Weight
    1001.9
  • Chemical structure
    Fura-2 AM  [108964-32-5]
  • Molecular Formula
    C44H47N3O24
  • CAS Number
    108964-32-5
  • PubChem identifier
    3364574
  • SMILES
    CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=C3C(=C2)C=C(O3)C4=NC=C(O4)C(=O)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
  • InChi
    InChI=1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,
  • InChiKey
    VPSRLGDRGCKUTK-UHFFFAOYSA-N
  • MDL number
    MFCD00036976
  • Appearance
    Yellow solid

Storing and Using Your Product

  • Storage instructions
    -20°C
  • Solubility overview
    Soluble in DMSO (10mM)
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for Fura-2 AM

  • Calcium imaging of cortical neurons using Fura-2 AM.

    Barreto-Chang OL et al (2009) J Vis Exp -23 :
    PubMedID: 19229178
  • Effects of transmitters and amyloid-beta peptide on calcium signals in rat cortical astrocytes: Fura-2AM measurements and stochastic model simulations.

    Toivari E et al (2011) PLoS One 6(3) : e17914.
    PubMedID: 21483471
  • Fura-2 measurement of cytosolic free Ca2+ in monolayers and suspensions of various types of animal cells.

    Malgaroli A et al (1987) J Cell Biol 105(5) : 2145-55.
    PubMedID: 3680375

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