Product overview

Name HEPES
Biological description

HEPES is a widely used cell culture buffering agent. It maintains physiological pH.

Description Buffering agent
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Solubility & Handling

Storage instructions Room temperature
Solubility overview Soluble in water (1000 mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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Chemical Data

Chemical name 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid
Molecular Weight 238.3
Chemical structure HEPES [7365-45-9] Chemical Structure
Molecular Formula C8H18N2O4S
CAS Number 7365-45-9
PubChem identifier 23830
SMILES OCCN1CCN(CCS(=O)(O)=O)CC1
InChi InChI=1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChiKey JKMHFZQWWAIEOD-UHFFFAOYSA-N
MDL number MFCD00006158

References for HEPES

References are publications that support the biological activity of the product
  • Effect of HEPES buffer systems upon the pH, growth and survival of Mycoplasma mycoides subsp. mycoides small colony (MmmSC) vaccine cultures

    Waite ER et al (2001) FEMS Microbiol Lett 201(2) : 291-4
  • Effects of bicarbonate versus HEPES buffering on measured properties of neurons in the salamander retina

    Hare WA et al (1998) Vis Neurosci 15(2) : 263-71
  • The effects of hepes buffer on clotting tests, assay of factors V and VIII and on the hydrolysis of esters by thrombin and thrombokinase

    Roberts PS et al (1976) Thromb Haemost 35(1) : 202-10

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