Janelia Fluor® 549, Tetrazine

(HB7228)
Technical documents: CoA Datasheet

Product overview

Name Janelia Fluor® 549, Tetrazine
Biological description

Cell-permeable, yellow fluorescent dye supplied with a tetrazine reactive handle for copper-free click chemistry. Can be coupled directly to a protein of interest by labeling unnatural amino acids with biorthogonal click chemistry in primary neurons. Suitable for confocal microscopy and super resolution microscopy (SRM) including techniques such as dSTORM (both live and fixed cells) and STED.


Spectrally similar dyes: Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3

Alternative names JF549-tetrazine
Purity >95%
Description

Yellow dye supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for dSTORM, STED, confocal microscopy and live cell imaging.

Write Your Own Review
You're reviewing:Janelia Fluor® 549, Tetrazine
Rate this item:

Optical Data

Reactivity Copper-free click chemistry
Emission color Yellow
Max excitation wavelength 549 nm
Max emission wavelength 571 nm
Quantum Yield (φ) 0.88
Extinction Coefficient (ε) 101,000 M-1cm-1
Correction Factor 280 0.169
Cell permeable Yes
Reactive Group Tetrazine

Solubility & Handling

Storage instructions -20°C
Solubility overview Soluble in DMSO
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

=
x
x
More Info

Dilution

x
=
x
More Info

Chemical Data

Purity >95%
Chemical name 3,6-Di-1-azetidinyl-9-[[4-[(1,2,4,5-tetrazin-3-yl)benzyl]carbamoyl]-2-carboxyphenyl]xanthylium, inner salt
Molecular Weight 623.66
Chemical structure Janelia Fluor® 549, Tetrazine Chemical Structure
Molecular Formula C36H29N7O4
SMILES [O-]C(=O)c1ccc(cc1C=1c2ccc(cc2OC2=CC(\C=CC=12)=[N+]1/CCC1)N1CCC1)C(=O)NCc1ccc(cc1)c1nncnn1
InChiKey LNBOAGLYGHAAER-UHFFFAOYSA-N
Appearance Purple solid

References for Janelia Fluor® 549, Tetrazine

References are publications that support the biological activity of the product
  • A general method to improve fluorophores for live-cell and single-molecule microscopy.

    Grimm JB et al (2015) Nature methods 12 : 244-50, 3 p following 250

1 Item