Product overview

  • Name
    Kainic acid
  • Short description
    Prototypic, selective kainate receptor agonist. Potent excitant and neurotoxin.
  • Biological description

    Prototypic, selective kainate receptor (KAR) agonist. Analog of L-Glutamate.


    Potent excitant and neurotoxin (shows ~30-fold more neurotoxic potency than L-Glutamate).

    Induces various changes in vivo including recurrent seizures, behavioural changes, oxidative stress, glial activation and selective neuronal death.


    Kainic acid exerts its neuroexcitatory action by binding to glutamate receptors to cause Ca2+ influx and triggering subsequent excitotoxic neuronal death pathway cascades.

    Kainic acid is widely used to model various neurodegenerative diseases and epilepsy.

  • Biological action
    Agonist
  • Customer comments

    We tested the Kainic acid and we are very happy with it. Hello Bio Kainic acid was used at 25 mg/kg i.p. and successfully induced status epilepticus. Verified customer, CNRS, France

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Properties

  • Chemical name
    (2S,3S,4S)-Carboxy-4-(1-methylethenyl)-3-pyrrolidineacetic acid
  • Molecular Weight
    213.23
  • Chemical structure
    Kainic acid  [487-79-6]
  • Purity
    >98%
  • Molecular Formula
    C10H15NO4
  • CAS Number
    487-79-6
  • PubChem identifier
    10255
  • SMILES
    CC(=C)[C@H]1CNC([C@H]1CC(=O)O)C(=O)O
  • Source
    Extracted from diginea simplex
  • InChi
    InChI=1S/C10H15NO4/c1-5(2)7-4-11-9(10(14)15)6(7)3-8(12)13/h6-7,9,11H,1,3-4H2,2H3,(H,12,13)(H,14,15)/t6-,7+,9-/m0/s1
  • InChiKey
    VLSMHEGGTFMBBZ-OOZYFLPDSA-N
  • MDL number
    MFCD00150833
  • Appearance
    White solid

Applications

  • Application notes

    The kainate receptor (KAR) agonist kainic acid induces neuronal oscillations in the gamma frequency band (35-100Hz). KAR activation leads to synchronous activity of excitatory and inhibitory network firing. Kainic acid is commonly used at concentrations of 400-600 nM to induce oscillations in acute brain slices. At a concentration of 550 nM, kainic acid from Hello Bio induces a strong gamma frequency neuronal oscillation (approx. 35Hz) within the CA3 region of the rat hippocampus (see Fig 1 above). Oscillations were abolished following kainic acid washout.

     

    #Protocol 1: Oscillations assay

    • Acute rat hippocampal slices were placed in an interface chamber and held at 30°C with a glass recording electrode placed in the CA3 region of the hippocampus.
    • Control basal activity was recorded for 1 hr in the presence of aCSF before continuous perfusion of aCSF containing kainic acid at 550nM to induce oscillations.
    • Oscillation activity was recorded once oscillations had stabilised (~2-3 hours). 
    • Kainic acid was then washed out and replaced with control aCSF. 
    • Data were analysed to create a power spectrum of the oscillation.

     

Storing and Using Your Product

  • Storage instructions
    Room temperature
  • Solubility overview
    Soluble in water (25mM, gentle warming)

References for Kainic acid

  • Kainic acid-mediated excitotoxicity as a model for neurodegeneration.

    Wang Q et al (2005) Mol Neurobiol 31(1-3) : 3-16.
    PubMedID: 15953808
  • Distinct modulation of the endocannabinoid system upon kainic acid-induced in vivo seizures and in vitro epileptiform bursting.

    Fezza F et al (2014) Mol Cell Neurosci 62 : 1-9.
    PubMedID: 25064144
  • Identification and characterization of the ligand binding subunit of a kainic acid receptor using monoclonal antibodies and peptide mapping.

    Hampson DR et al (1989) J Biol Chem 264(22) : 13329-35.
    PubMedID: 2546953
  • Pharmacological characterization of glutamatergic agonists and antagonists at recombinant human homomeric and heteromeric kainate receptors in vitro.

    Alt et al (2004) Neuropharmacology 46(6) : 793-806
    PubMedID: 15033339

Reviews

  • Verified customer, CNRS, France

    We tested the Kainic acid and we are very happy with it. Hello Bio Kainic acid was used at 25 mg/kg i.p. and successfully induced status epilepticus.