Potent, selective, competitive GABAA receptor agonist
Potent, selective and competitive GABAA receptor agonist and a potent partial GABAA-ρ (GABAC) receptor agonist.
Muscimol is a GABA analog with comparable potency to GABA and is thought to act at the orthosteric site at GABAA receptors in varying active conformations.
Also acts as a weak inhibitor of GABA uptake but is not a substrate for GABA transaminase.
Muscimol enhances inhibitory neurotransmission and suppresses spontaneous activity. It is commonly used in reversible brain inactivation studies.
Active in vivo and blood brain barrier permeable.
Shows psychoactive, memory impairing effects and anticonvulsant actions at high doses.
1 citation Submit Your Citation Now
The GABAA receptor agonist muscimol is used at concentrations of 1-50 µM. Muscimol from Hello Bio used at 10 µM led to a large hyperpolarising whole-cell current in hippocampal CA1 neurons (see Fig 1 above). Action of muscimol was fully blocked by the GABAA receptor antagonist bicuculline (100 µM).
#Protocol 1: Assay used for muscimol
- Whole cell voltage clamp recordings of CA1 pyramidal neurons from the rat hippocampal brain slice.
- Neurons were held at 0 mV and GABAA receptor currents were evoked via applying muscimol directly to the recording chamber during continuous perfusion.
- To test muscimol’s selectivity to GABAA receptors the experiment was repeated within the same neuron in the presence of the GABAA receptor antagonist bicuculline (100 µM).
- Under these conditions muscimol failed to induce a hyperpolarising current.
Storing and Using Your Product
Soluble in water (100mM)
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
References for Muscimol
Anticonvulsant and behavioral effects of muscimol in immature rats.Mareš P et al (2014) Brain Res 1582 : 227-36.PubMedID: 25084038
Muscimol as an Ionotropic GABA Receptor Agonist.Johnston GA (2014) Neurochem Res 39(10) : 1942-7.PubMedID: 24473816
Hippocampal infusions of pyruvate reverse the memory-impairing effects of septal muscimol infusions.Krebs DL et al (2005) Eur J Pharmacol 520(1-3) : 91-9.PubMedID: 16150437
mGluR-LTD at Excitatory and Inhibitory Synapses in the Lateral Habenula Tunes Neuronal Output.Valentinova and Mameli(2016) Cell Rep 16(9) : 2298-307PubMedID: 27545888