Product overview

  • Name
    Muscimol
  • Short description
    Potent, selective, competitive GABAA receptor agonist
  • Biological description

    Potent, selective and competitive GABAA receptor agonist and a potent partial GABAA-ρ (GABAC) receptor agonist.


    Muscimol is a GABA analog with comparable potency to GABA and is thought to act at the orthosteric site at GABAA receptors in varying active conformations.


    Also acts as a weak inhibitor of GABA uptake but is not a substrate for GABA transaminase.


    Application of muscimol evokes GABAAR currents and its actions are antagonized by the GABAAR antagonist bicuculline (bicuculline methochloride, methiodide and methobromide also available).

    Muscimol enhances inhibitory neurotransmission and suppresses spontaneous activity. It is commonly used in reversible brain inactivation studies.


    Active in vivo and blood brain barrier permeable.

    Shows psychoactive, memory impairing effects and anticonvulsant actions at high doses.

  • Biological action
    Agonist
  • Purity
    >99%
  • Our products in action

Images

Properties

  • Chemical name
    5-Aminomethyl-3-hydroxyisoxazole
  • Molecular Weight
    114.1
  • Chemical structure
    Muscimol  [2763-96-4]
  • Molecular Formula
    C4H6N2O2
  • CAS Number
    2763-96-4
  • PubChem identifier
    4266
  • SMILES
    C1=C(ONC1=O)CN
  • Source
    Synthetic
  • InChi
    InChI=1S/C4H6N2O2/c5-2-3-1-4(7)6-8-3/h1H,2,5H2,(H,6,7)
  • InChiKey
    ZJQHPWUVQPJPQT-UHFFFAOYSA-N
  • MDL number
    MFCD00057894
  • Appearance
    White solid

Applications

  • Application notes

    The GABAA receptor agonist muscimol is used at concentrations of 1-50 µM. Muscimol from Hello Bio used at 10 µM led to a large hyperpolarising whole-cell current in hippocampal CA1 neurons (see Fig 1 above). Action of muscimol was fully blocked by the GABAA receptor antagonist bicuculline (100 µM).


    #Protocol 1: Assay used for muscimol

    • Whole cell voltage clamp recordings of CA1 pyramidal neurons from the rat hippocampal brain slice.
    • Neurons were held at 0 mV and GABAA receptor currents were evoked via applying muscimol directly to the recording chamber during continuous perfusion.
    • To test muscimol’s selectivity to GABAA receptors the experiment was repeated within the same neuron in the presence of the GABAA receptor antagonist bicuculline (100 µM).
    • Under these conditions muscimol failed to induce a hyperpolarising current.

Storing and Using Your Product

  • Storage instructions
    Room temperature
  • Solubility overview
    Soluble in water (100mM)

References for Muscimol

  • Anticonvulsant and behavioral effects of muscimol in immature rats.

    Mareš P et al (2014) Brain Res 1582 : 227-36.
    PubMedID: 25084038
  • Muscimol as an Ionotropic GABA Receptor Agonist.

    Johnston GA (2014) Neurochem Res 39(10) : 1942-7.
    PubMedID: 24473816
  • Hippocampal infusions of pyruvate reverse the memory-impairing effects of septal muscimol infusions.

    Krebs DL et al (2005) Eur J Pharmacol 520(1-3) : 91-9.
    PubMedID: 16150437
The following papers have cited the use of Muscimol (HB0887) from Hello Bio. If you have published a paper using this product, and it is not shown here, then please tell us! We will send you a free gift as a thank you!
  • mGluR-LTD at Excitatory and Inhibitory Synapses in the Lateral Habenula Tunes Neuronal Output.

    Valentinova and Mameli(2016) Cell Rep 16(9) : 2298-307
    PubMedID: 27545888

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