Catalyzes ADP-ribosylation of the G proteins Gαi, Gαo and Gαt
Toxin produced by Bordetella pertussis . Catalyzes ADP-ribosylation of the α subunits of the heterotrimeric Gi/o proteins; Gαi, Gαo and Gαt . Blocks receptor coupling and activation by preventing G protein heterotrimers from interacting with receptors.
This product is not activated. Cells will activate pertussis toxin in an intact system however activation is required in a cell free system. Incubation with high concentrations of dithiothreitol (DTT) can achieve activation, see Kaslow, et al. (1987) for suggested conditions.
PTX | PT | Islet-activating protein | Holotoxin
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Contains 0.05M sodium phosphate and 0.5M sodium chloride at pH 7.2.
Storing and Using Your Product
+4°C for lyophilised or resuspended (do not freeze).
Use solutions within 1 month, long term storage is not recommended.
Before use the suspension should be gently mixed (not vortexed) to make the suspension uniform. Do not sterile filter.
Pertussis toxin can be inactivated by boiling for 30mins
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
References for Pertussis Toxin
Subunit structure of islet-activating protein, pertussis toxin, in conformity with the A-B model.Tamura et al (1982) Biochemistry 21(22) : 5516-22.PubMedID: 6293544
Structure-activity analysis of the activation of pertussis toxin.Kaslow et al (1987) Biochemistry 26(1) : 123-7PubMedID: 3030399
G(i/o) protein-dependent and -independent actions of Pertussis Toxin (PTX).Mangmool and Kurose (2011) Toxins (Basel) 3(7) : 884-99PubMedID: 22069745
Cannabichromene is a cannabinoid CB2 receptor agonistUdoh et al (2018) bioRxiv doi: : https://doi.org/10.1101/435057