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Propidium Iodide

(HB0820)
Data

Product overview

  • Name
    Propidium Iodide
  • Short description
    Red-fluorescent cell viability dye
  • Biological description

    Propidium iodide (PI) is a widely used red-fluorescent intercalating agent that binds and labels nucleic acids.


    Propidium iodide is membrane impermeant and is therefore frequently used to selectively identify dead cells and is commonly used in flow cytometry to evaluate cell viability.


    Propidium iodide (PI) is often used in flow cytometry, fluorescent microscopy and confocal laser scanning microscopy applicaitons.


    Wavelength Maxima: Excitation 535nm, Emission 617nm

  • Biological action
    Dyes & stains
  • Purity
    >95%
  • Citations

Properties

  • Chemical name
    2,7-Diamino-9-phenyl-10 (diethylaminopropyl)-phenanthridium iodide methiodide
  • Molecular Weight
    668.39
  • Chemical structure
    Propidium Iodide  [25535-16-4]
  • Molecular Formula
    C27H34I2N4
  • CAS Number
    25535-16-4
  • PubChem identifier
    104981
  • SMILES
    CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-]
  • InChi
    InChI=1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
  • InChiKey
    XJMOSONTPMZWPB-UHFFFAOYSA-M
  • MDL number
    MFCD00011921
  • Appearance
    Purple solid

Storing and Using Your Product

  • Storage instructions
    +4°C
  • Solubility overview
    Soluble in water (5 mM)
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for Propidium Iodide

  • Analysis of apoptosis by propidium iodide staining and flow cytometry.

    Riccardi C et al (2006) Nat Protoc 1(3) : 1458-61.
    PubMedID: 17406435
  • The DNA intercalators ethidium bromide and propidium iodide also bind to core histones.

    Banerjee A et al (2014) FEBS Open Bio 4 : 251-9.
    PubMedID: 24649406
  • DNA staining for fluorescence and laser confocal microscopy.

    Suzuki T et al (1997) J Histochem Cytochem 45(1) : 49-53.
    PubMedID: 9010468
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