AMPA receptor agonist
(S)-AMPA is an AMPA receptor agonist and the active enantiomer of AMPA. It is a neurotoxin in the immature rat brain.
(R,S)-AMPA is also available.
The AMPA receptor agonist (S)-AMPA is typically used at concentrations of 1-100 µM. At 10 µM, (S)-AMPA from Hello Bio induces a large depolarising current. This depolarising current was occluded in the presence of the AMPA receptor antagonist NBQX (20µM). (See Fig 1 above).
#Protocol 1: (S)-AMPA protocol
- Whole cell voltage clamp recordings of CA1 pyramidal neurons from the rat hippocampal brain slice.
- Neurons were held at -60 mV and continuously perfused with aCSF in the presence of the GABA receptor antagonist gabazine (20µM).
- AMPA currents were evoked via applying (S)-AMPA directly to the recording chamber during continuous perfusion.
- To test the selectivity of (S)-AMPA to AMPA receptors, the experiment was repeated within the same neuron in the presence of the AMPA receptor antagonist NBQX (20 µM)
- Under these conditions (S)-AMPA failed to induce a depolarising current.
Storing and Using Your Product
Soluble in water (100mM)
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
References for (S)-AMPA
Enzymic resolution and binding to rat brain membranes of the glutamic acid agonist alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid.Hansen JJ et al (1983) J Med Chem 26(6) : 901-3.PubMedID: 6133955
Ibotenic acid analogues. Synthesis, molecular flexibility, and in vitro activity of agonists and antagonists at central glutamic acid receptors.Lauridsen J et al (1985) J Med Chem 28(5) : 668-72.PubMedID: 2859375
The selective ionotropic-type quisqualate receptor agonist AMPA is a potent neurotoxin in immature rat brain.McDonald JW et al (1990) Brain Res 526(1) : 165-8.PubMedID: 1964108