SP600125

(HB2234)

Product overview

  • Name
    SP600125
  • Short description
    Selective, ATP-competitive JNK inhibitor. Prevents BMP9-induced osteogenic MSC differentiation.
  • Biological description

    SP600125 is a selective, cell-permeable, reversible and ATP-competitive JNK (c-Jun N-terminal Kinase) inhibitor (IC50 values are 40-90 µM at JNK1,2,3). SP600125 shows selectivity over a range of other kinases.


    SP600125 dose-dependently inhibits phosphorylation of JNK, inhibits expression of the inflammatory genes (COX-2, IL-2, IFN-γ and TNF-α) and prevents apoptosis in many cell types and inhibits autophagy in HeLa cells.


    In addition, SP600125 also shows neuronal protective effects and is active in vivo.


    SP600125 is also commonly used as a stem cell modulator which prevents BMP9-induced osteogenic mesenchymal stem cells (MSCs) and primary bone marrow stromal cells differentiation and is frequently used in media to maintain stem cells in naive pluripotent state.

  • Biological action
    Inhibitor
  • Purity
    >98%
  • Citations

Properties

  • Chemical name
    Anthra[1-9-cd]pyrazol-6(2H)-one
  • Molecular Weight
    220.23
  • Chemical structure
    SP 600125 [129-56-6]
  • Molecular Formula
    C14H8N2O
  • CAS Number
    129-56-6
  • PubChem identifier
    8515
  • SMILES
    C1=CC=C2C(=C1)C3=NNC4=CC=CC(=C43)C2=O
  • InChi
    InChI=1S/C14H8N2O/c17-14-9-5-2-1-4-8(9)13-12-10(14)6-3-7-11(12)15-16-13/h1-7H,(H,15,16)
  • InChiKey
    ACPOUJIDANTYHO-UHFFFAOYSA-N
  • MDL number
    MFCD00022289
  • Appearance
    Yellow solid

Storing and Using Your Product

  • Storage instructions
    -20°C (desiccate)
  • Solubility overview
    soluble in DMSO (100mM) or ethanol (5mM, gentle warming)
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for SP600125

  • SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase.

    Bennett BL et al (2001) Proc Natl Acad Sci U S A 98(24) : 13681-6.
    PubMedID: 11717429
  • SP600125, a selective JNK inhibitor, protects ischemic renal injury via suppressing the extrinsic pathways of apoptosis.

    Wang Y et al (2007) Life Sci 80(22) : 2067-75.
    PubMedID: 17459422
  • SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase.

    Bennett BL et al (2001) Proc Natl Acad Sci U S A 98(24) : 13681-6.
    PubMedID: 11717429
  • Differential Regulation of Evoked and Spontaneous Release by Presynaptic NMDA Receptors

    Abrahamsson et al (2017) Neuron 09 : 030
The following papers have cited the use of SP600125 (HB2234) from Hello Bio. If you have published a paper using this product, and it is not shown here, then please tell us! We will send you a free gift as a thank you!
  • Differential Regulation of Evoked and Spontaneous Release by Presynaptic NMDA Receptors

    Abrahamsson et al(2017) Neuron 09 : 030