WinLTP - data acquisition program
Standard LTP/LTD experiments
- Alternating extracellular pathway stimulation
- Online signal averaging and filtering
- LTP induction by single trains and theta burst stimulation
- LTD induction by low frequency stimulation
- PopSpike measurements
The program is easy to use - after installation, a single mouse click starts the baseline recording of an extracellular LTP experiment with averaging, and a single mouse click evokes single train stimulation, theta burst stimulation, or LowFrequencyStimulation (for LTD induction).
Multitasking and continuous acquisition
WinLTP can simultaneously run a continuous acquisition task (acquiring a gap-free ABF, top trace) and a sweep stimulation/acquisition task (bottom trace, here measuring patch electrode series resistance and cell resistance). This is done using one data acquisition board.
The Protocol Builder adds flexibility enabling automated perfusion (below) and more complicated stimulation induction protocols such as this in vitro epilepsy kindling protocol (right).
Automated experiments are performed by coupling automated perfusion control with the Protocol Builder’s normal sweep stimulation/acquisition protocol. This enables multi-slice / multi-chamber experiments to be more easily performed, and each chamber can have its own perfusion and stimulation protocol.
Imposing single and averaged sweeps
Single sweeps can be imposed during acquisition and reanalysis just by double-clicking on an Analysis graph data point (right, arrow to purple trace). This is useful for seeing what part of the sweep waveform caused an outlier data point miscalculation. Imposing an average of many sweeps can also be performed during acquisition and reanalysis (left). This is useful for comparing the baseline averaged waveform to the current waveform.
The ease of reanalyzing data has been dramatically improved in WinLTP 2.10/2.20. This includes quarantining (ie removing) noisy sweeps during signal averaging, automatically decreasing the number of sweeps in the averaged group., and recalculating PkAmp, etc.
The upcoming WinLTP 2.30 will include software-only 50/60 Hz hum cancellation during evoked sweeps. Initially this will be for only National Instruments boards, 50 Hz mains frequency, and no spontaneous activity.
WinLTP uses National Instruments M- and X-Series boards and the Molecular Devices’ Digidata 1322A.
WinLTP is available in two versions: the Standard Version has full capabilities except automated perfusion, and the Advanced Version includes automated perfusion. A free two month trial is available.