NameROCK inhibitor Y-27632
Short descriptionY-27632 is a selective ROCK inhibitor
The ROCK inhibitor Y-27632 is a selective and cell permeable inhibitor of the Rho-associated protein kinase (ROCK) (Ki values are 0.14, 26 ,25 and >250 µM at p160ROCK, PKC, PKA and MLCK respectively).
Y-27632 has many biological actions and is an important small molecule modulator of stem cells. It diminishes hES and hiPSC dissociation-induced apoptosis, enhances survival and colony formation of dissociated hESC without affecting pluripotency or self-renewal.
Y-27632 also improves hESC and iPSCs postthaw viability and survival rate during cryopreservation.
Additionally, Y-27632 inhibits smooth muscle contractility and shows antihypertensive effect.
Alternative namesY27632 dihydrochloride
The ROCK inhibitor Y-27632 is working as expected. Verified customer, University of Louisville
Chemical nametrans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride
Rho kinase (Rho-associated coiled coil protein kinase or ROCK) is a major downstream effector for the small GTP-binding protein Rho . Rho kinase is involved in various biological processes including cell mitosis adhesion, cytoskeletal adjustments, muscle cell contraction, tumor cell invasion and a series of cell biological phenomena . Through a series of cascading effects, Rho kinase influences actin and microtubule regulation of apoptosis and thus plays a key role in early embryonic development .The Rho kinase signalling pathway is highly activated in many pathological conditions .
Rock inhibitor Y-27632 is a Rho kinase inhibitor which selectively inhibits p160ROCK (ROCK1) with a Ki value of 140 nM and binds to the Rho kinase ATP binding pocket in an ATP-competitive manner .
UsesY-27632 has been shown to have a diverse range of actions and has been used in a variety of biological systems. It is the most commonly used of the ROCK inhibitors in stem cell research and is used as a multifunctional reagent in many stem cell related processes . Y-27632 demonstrates many positive effects across various cell types, in particular human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs)  and has greatly improved a number of practical stem cell procedures . For example it has been shown that Y-27632 increases cell adhesion and proliferation and increases post-thaw cell survival and post-passaging cell viability [9,10].
Cell dissociation is one of the most common manipulations in stem cell research. During cellular dissociation both hESC and hIPSCs are vulnerable to dissociation-induced apoptosis or anoikis (a form of apoptosis induced by inappropriate cell-cell or cell-ECM interactions) [3,11]. Y-27632 is of particular interest as it allows hPSCs to escape this dissociation-induced apoptosis. Y-27632 prevents apoptosis of dissociated hPSCs and increases their survival rate and plating efficiency .
Cryopreservation is also an important part of stem cell research. Y-27632 improves the recovery of cryopreserved hPSCs and has greatly helped simplify the cryopreservation procedure. The slow-freezing and rapid-thawing procedure which uses DMSO as a cryoprotectant is commonly used in stem cell cryopreservation, however this method is not suitable for hPSCs, therefore vitrification (fast freezing, slow thawing) is used . Vitrified hPSCs do however still suffer from high levels of cell death and do not passage well as single cells . The addition of Y-27632 to freezing and post-thawing medium enhances colony formation efficiency and treatment with Y-27632 during cryopreservation also increases survival rate and cell adhesion of freeze-thawed dissociated hES .
Storing and Using Your Product
Storage instructionsRoom temperature (desiccate)
Solubility overviewSoluble in water (100mM)
ImportantThis product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
References for ROCK inhibitor Y-27632
1, Rho-kinase/ROCK: A key regulator of the cytoskeleton and cell polarity.Amano et al (2010) Cytoskeleton (Hoboken) 67(9) : 545-54PubMedID: 20803696
2, Rocks: multifunctional kinases in cell behaviour.Riento and Ridley (2003) Nat Rev Mol Cell Biol 4(6) : 446-56PubMedID: 12778124
3, ROCK inhibitor Y27632 promotes proliferation and diminishes apoptosis of marmoset induced pluripotent stem cells by suppressing expression and activity of caspase 3.Wu et al (2016) Theriogenology 85(2) : 302-14PubMedID: 26476594
4, Detrimental effect of post Status Epilepticus treatment with ROCK inhibitor Y-27632 in a pilocarpine model of temporal lobe epilepsy.Kourdougli et al (2015) Front Cell Neurosci. 9 : :413PubMedID: https://www.ncbi.nlm.nih.gov/pub
5, Protein kinase A in complex with Rho-kinase inhibitors Y-27632, Fasudil, and H-1152P: structural basis of selectivity.Breitenlechner et al (2003) Structure 11(12) : 1595-607PubMedID: 14656443
6, Reprogram or reboot: small molecule approaches for the production of induced pluripotent stem cells and direct cell reprogramming.Jung et al (2014) ACS Chem Biol 9(1) : 80-95PubMedID: 24245936
7, A ROCK inhibitor permits survival of dissociated human embryonic stem cells.Wanatabe et al (2007) Nat Biotechnol 25(6) : 681-6PubMedID: 17529971
8,Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells.Ohgushi et al (2010) Cell Stem Cell 7(2) : 225-39PubMedID: 20682448
9, Failure of Y-27632 to improve the culture of adult human adipose-derived stem cells.Lamas et al (2015) Stem Cells Cloning 8 : 15-26PubMedID: 25609984
10, Effect of Rho-kinase Inhibitor, Y27632, on Porcine Corneal Endothelial Cell Culture, Inflammation and Immune Regulation.Lee et al (2016) Ocul Immunol Inflamm 24(5) : 579-93PubMedID: 26471144
11, Application of Rho-associated protein kinase (ROCK) inhibitor to human pluripotent stem cells.Kurosawa (2012) J Biosci Bioeng. 114(6) : 577-81PubMedID: 22898436
12, Novel cryopreservation method for dissociated human embryonic stem cells in the presence of a ROCK inhibitor.Martin-Ibañez et al (2008) Hum Reprod. 23(12) : 2744-54PubMedID: 18716037
- The Y-27632 is working as expected.