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ROCK inhibitor Y-27632


Product overview

  • Name
    ROCK inhibitor Y-27632
  • Short description
    Y-27632 is a selective ROCK inhibitor
  • Biological description

    The ROCK inhibitor Y-27632 is a selective and cell permeable inhibitor of the Rho-associated protein kinase (ROCK) (Ki values are 0.14, 26 ,25 and >250 µM at p160ROCK, PKC, PKA and MLCK respectively).

    Y-27632 has many biological actions and is an important small molecule modulator of stem cells. It diminishes hES and hiPSC dissociation-induced apoptosis, enhances survival and colony formation of dissociated hESC without affecting pluripotency or self-renewal.

    Y-27632 also improves hESC and iPSCs postthaw viability and survival rate during cryopreservation.

    Additionally, Y-27632 inhibits smooth muscle contractility and shows antihypertensive effect. 

  • Alternative names
    Y27632 dihydrochloride
  • Biological action
  • Purity
  • Customer comments

    The ROCK inhibitor Y-27632 is working as expected. Verified customer, University of Louisville

    Worked well just as it should do. Kept me informed of when product would be delivered and notified me when delivered. Good packaging and data sheet. Easy to dissolve and use and it worked! Verified customer, University of Cardiff

  • Citations



  • Chemical name
    trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride
  • Molecular Weight
  • Chemical structure
    Y-27632 dihydrochloride [129830-38-2]
  • Molecular Formula
  • CAS Number
  • PubChem identifier
  • Source
  • InChi
  • InChiKey
  • MDL number
  • Appearance
    White solid


  • Application notes


    Rho kinase (Rho-associated coiled coil protein kinase or ROCK) is a major downstream effector for the small GTP-binding protein Rho [1]. Rho kinase is involved in various biological processes including cell mitosis adhesion, cytoskeletal adjustments, muscle cell contraction, tumor cell invasion and a series of cell biological phenomena [2]. Through a series of cascading effects, Rho kinase influences actin and microtubule regulation of apoptosis and thus plays a key role in early embryonic development [3].The Rho kinase signalling pathway is highly activated in many pathological conditions [4].

    Rock inhibitor Y-27632 is a Rho kinase inhibitor which selectively inhibits p160ROCK (ROCK1) with a Ki value of 140 nM and binds to the Rho kinase ATP binding pocket in an ATP-competitive manner [5].


    Y-27632 has been shown to have a diverse range of actions and has been used in a variety of biological systems. It is the most commonly used of the ROCK inhibitors in stem cell research and is used as a multifunctional reagent in many stem cell related processes [6]. Y-27632 demonstrates many positive effects across various cell types, in particular human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) [7] and has greatly improved a number of practical stem cell procedures [8]. For example it has been shown that Y-27632 increases cell adhesion and proliferation and increases post-thaw cell survival and post-passaging cell viability [9,10].

    Cellular dissociation

    Cell dissociation is one of the most common manipulations in stem cell research. During cellular dissociation both hESC and hIPSCs are vulnerable to dissociation-induced apoptosis or anoikis (a form of apoptosis induced by inappropriate cell-cell or cell-ECM interactions) [3,11]. Y-27632 is of particular interest as it allows hPSCs to escape this dissociation-induced apoptosis. Y-27632 prevents apoptosis of dissociated hPSCs and increases their survival rate and plating efficiency [11].


    Cryopreservation is also an important part of stem cell research. Y-27632 improves the recovery of cryopreserved hPSCs and has greatly helped simplify the cryopreservation procedure. The slow-freezing and rapid-thawing procedure which uses DMSO as a cryoprotectant is commonly used in stem cell cryopreservation, however this method is not suitable for hPSCs, therefore vitrification (fast freezing, slow thawing) is used [12]. Vitrified hPSCs do however still suffer from high levels of cell death and do not passage well as single cells [11]. The addition of Y-27632 to freezing and post-thawing medium enhances colony formation efficiency and treatment with Y-27632 during cryopreservation also increases survival rate and cell adhesion of freeze-thawed dissociated hES [12].

Storing and Using Your Product

  • Storage instructions
    Room temperature (desiccate)
  • Solubility overview
    Soluble in water (100mM), PBS (100mM), DMSO (100mM)
  • Handling
    • If using the compound as a cell culture supplement, you should dilute the stock solution into culture medium immediately before use.
    • Cell culture media can be prewarmed before adding the compound to avoid potential precipitaion of the compound.
    • Y-2762 is often removed from medium within 20 hours
    • The compound has been shown to be effective at a final concentration of 10 µM (Ungrin et al.; Watanabe et al.)
    • Please avoid a final DMSO concentration above 0.1% due to potential cell toxicity
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for ROCK inhibitor Y-27632

  • 1, Rho-kinase/ROCK: A key regulator of the cytoskeleton and cell polarity.

    Amano et al (2010) Cytoskeleton (Hoboken) 67(9) : 545-54
    PubMedID: 20803696
  • 2, Rocks: multifunctional kinases in cell behaviour.

    Riento and Ridley (2003) Nat Rev Mol Cell Biol 4(6) : 446-56
    PubMedID: 12778124
  • 3, ROCK inhibitor Y27632 promotes proliferation and diminishes apoptosis of marmoset induced pluripotent stem cells by suppressing expression and activity of caspase 3.

    Wu et al (2016) Theriogenology 85(2) : 302-14
    PubMedID: 26476594
  • 4, Detrimental effect of post Status Epilepticus treatment with ROCK inhibitor Y-27632 in a ... model of temporal lobe epilepsy.

    Kourdougli et al (2015) Front Cell Neurosci. 9 : :413
  • 5, Protein kinase A in complex with Rho-kinase inhibitors Y-27632, Fasudil, and H-1152P: structural basis of selectivity.

    Breitenlechner et al (2003) Structure 11(12) : 1595-607
    PubMedID: 14656443
  • 6, Reprogram or reboot: small molecule approaches for the production of induced pluripotent stem cells and direct cell reprogramming.

    Jung et al (2014) ACS Chem Biol 9(1) : 80-95
    PubMedID: 24245936
  • 7, A ROCK inhibitor permits survival of dissociated human embryonic stem cells.

    Wanatabe et al (2007) Nat Biotechnol 25(6) : 681-6
    PubMedID: 17529971
  • 8,Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells.

    Ohgushi et al (2010) Cell Stem Cell 7(2) : 225-39
    PubMedID: 20682448
  • 9, Failure of Y-27632 to improve the culture of adult human adipose-derived stem cells.

    Lamas et al (2015) Stem Cells Cloning 8 : 15-26
    PubMedID: 25609984
  • 10, Effect of Rho-kinase Inhibitor, Y27632, on Porcine Corneal Endothelial Cell Culture, Inflammation and Immune Regulation.

    Lee et al (2016) Ocul Immunol Inflamm 24(5) : 579-93
    PubMedID: 26471144
  • 11, Application of Rho-associated protein kinase (ROCK) inhibitor to human pluripotent stem cells.

    Kurosawa (2012) J Biosci Bioeng. 114(6) : 577-81
    PubMedID: 22898436
  • 12, Novel cryopreservation method for dissociated human embryonic stem cells in the presence of a ROCK inhibitor.

    Martin-Ibañez et al (2008) Hum Reprod. 23(12) : 2744-54
    PubMedID: 18716037
Support & Resources


  • Verified customer

    Works just as intended, at half the price of what it costs at another company..
  • Verified customer, University of Cardiff

    Worked well just as it should do. Kept me informed of when product would be delivered and notified me when delivered. Good packaging and data sheet. Easy to dissolve and use and it worked!
  • Verified customer, University of Louisville

    The Y-27632 is working as expected.