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DREADD agonist 21 (Compound 21) dihydrochloride (water soluble)


Product overview

  • Name
    DREADD agonist 21 (Compound 21) dihydrochloride (water soluble)
  • Short description
    Effective agonist for muscarinic-based DREADDs in vitro and in vivo. Non-CNO chemogenetic actuator. Brain penetrant. Water soluble.
  • Biological description


    DREADD agonist 21 (Compound 21) hydrochloride is a water soluble salt of DREADD agonist 21 (Compound 21)  which represents an alternative chemogenetic actuator for muscarinic -based DREADDs and an alternative to CNO for in vivo studies in which metabolic conversion of CNO to clozapine is an issue.

    DREADD agonist 21 (Compound 21) has excellent bioavailability, pharmacokinetic properties and brain penetrability. It has been reported that the compound does not undergo back metabolism to clozapine.

    DREADD agonist 21 is a potent and selective agonist at muscarinic based DREADDs such as the excitatory hM3Dq, hM1Dq and inhibitory hM4Di DREADDs (pEC50 values are 8.48, 8.91 and 7.77 at hM3Dq, hM1Dq and hM4Di respectively). The compound exhibits >10-fold higher affinity at hM1Dq and hM4Di DREADDs compared to wild type receptors and also lacks agonist activity at wild type receptors.

    In vivo use

    DREADD agonist 21 from Hello Bio has recently been cited in a pharmacokinetic profile of the compound in mice by Jendryka et al (2019) which suggests that DREADD agonist 21 shows superior brain penetration and long-lasting presence. They suggest that the compound is a suitable DREADD agonist which is effective at latest 15 min after i.p injection, but requires between-subject controls for unspecific effects.

    Pharmacokinetic profile of DREADD agonist 21

    Figure: In vivo pharmacokinetic profile of DREADD agonist 21 (Cmpd-21). DREADD agonist 21 was from Hello Bio.

    (j–l): Concentration (nM) of Cmpd-21 (blue) at 15, 30 and 60 min after i.p. injection of 3.0 mg/kg Cmpd-21 in (j) plasma, (k) CSF, and (l) cortical brain tissue. Reproduced from Jendryka et al Sci Rep. 2019;9(1):4522

    DREADD agonist 21 (Compound 21)-induced activation of hM3Dq and hM4Di can modulate bi-directional feeding in defined circuits in mice. Concentrations of DREADD agonist 21 that resulted in changes in feeding behavior in animals expressing muscarinic DREADDs had no off-target effects in control animals (where musarinic DREADDs were not expressed).

    Off target binding

    DREADD agonist 21 shows weak to moderate binding affinity at a range of wild type GPCRs which may translate to functional antagonism in vivo.

    Therefore care should be taken with in vivo dosing of DREADD agonist 21 to ensure the free concentration of the compound remains in a range that activates muscarinic DREADDs but is sufficiently low to avoid antagonism at wild type GPCRs.

    Strong competitive binding of DREADD agonist 21 (Cmpd-21) to receptor sites of dopamine, 5-HT, opioid, muscarinic, histamine and adrenoceptors in mice has been shown. Only very subtle, if any behavioural alterations using the 5-CSRTT assessment were found Jendryka et al (2019).

    In vivo experiments should be conducted with the appropriate controls where DREADD agonist 21 is administered to animals that do not express the muscarinic-DREADDs.

    Data from a poster presented at SfN by the Michaelides group indicates that DREADD agonist 21 (Compound 21) has lower binding affinity for DREADDs compared to clozapine and that DREADD agonist 21 induces behavioural effects in transgenic mice. 1mg/kg doses were used. High doses (10mg/kg) may cause sedation.

  • Alternative names
  • Purity
  • Customer comments

    We used Compound 21 (DREADD agonist 21) dihydrochloride (water soluble) (1mg/kg) and CNO in rats (1mg/kg) and we found a great effect on cells infected with dreadds-Gs. Both products are really easy to dissolve in saline! Verified customer, Universite de Bordeaux

    Great Product!, Product was delivered promptly. Very easy to work with. No problems keeping it in solution. Verified customer, Florida Atlantic University

  • Citations



  • Chemical name
    11-(1-Piperazinyl)-5H-dibenzo[b,e][1,4]diazepine dihydrochloride
  • Molecular Weight
  • Chemical structure
    Compound 21 hydrochloride
  • Molecular Formula
  • Source
  • InChi
  • InChiKey
  • Appearance
    Yellow solid

Storing and Using Your Product

  • Storage instructions
  • Solubility overview
    Soluble in water (100mM). Always store solutions at -20°C.
  • Handling

    Storage of solid

    • Store at -20°C.
    • Please note that the compound is a hydroscopic solid and contact with air may cause material to become sticky. Product performance should not be affected but we recommend storing the material in a sealed jar.

    Storage of solutions

    • Make up solutions and use immediately.
    • If storage of solutions is required, you should aliquot out the solution into tightly sealed vials and store at -20°C and store these for up to one month.
    • Allow the product to equilibrate to RT for at least one hour before opening and using.
  • Handling continued..

    Storage of solutions at room temperature

    • We recommend only keeping solutions at room temperature (25°C) for a few days as our studies have shown that after 96 hours the purity of the compound in solution drops to ~95% and will continue to drop over time.
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

References for DREADD agonist 21 (Compound 21) dihydrochloride (water soluble)

  • The first structure-activity relationship studies for designer receptors exclusively activated by designer drugs.

    Chen et al (2015) ACS Chem Neurosci 6(3) : 476-84
    PubMedID: 25587888
  • Optogenetic approaches for dissecting neuromodulation and GPCR signaling in neural circuits.

    Spangler and Bruchas (2017) Curr Opin Pharmacol 32(4) : 56-70.
    PubMedID: 27875804
  • Clozapine N-Oxide Administration Produces Behavioral Effects in Long-Evans Rats: Implications for Designing DREADD Experiments.

    MacLaren et al (2016) eNeuro 3(5) : 0219-16
    PubMedID: 27822508
  • New non-CNO actuators for DREADDs

    Roth Bl (2015) Blog : N/A
  • DREADD Agonist 21 Is an Effective Agonist for Muscarinic-Based DREADDs in Vitro and in Vivo

    Thompson et al (2018) ACS Pharmacol. Transl. Sci. 10.1021 : /acsptsci.8b00012
  • Pharmacokinetic and pharmacodynamic actions of clozapine-N-oxide, clozapine, and compound 21 in DREADD-based chemogenetics in mice.

    Jendryka et al (2019) Sci Rep. 9(1) : 4522
    PubMedID: 30872749
Our products in action
The following papers have cited the use of DREADD agonist 21 (Compound 21) dihydrochloride (water soluble) (HB6124) from Hello Bio. If you have published a paper using this product, and it is not shown here, then please tell us! We will send you a free gift as a thank you!
  • Effects of Chemogenetic Inhibition of Dopamine Transporter-or A2A-Expressing Neurons On Spontaneous Activity and Motivation to Consume a Palatable Food

    Wherry(2018) Binghamton University : Thesis
  • Astrocytes Integrate Behavioral State and Vascular Signals during Functional Hyperemia

    Tran et al (2018) Neuron doi: :
  • Convergent inputs from the hippocampus and thalamus to the nucleus accumbens regulate dopamine neuron activity

    Perez and Lodge(2018) J Neurosci : 2629-16
  • Olanzapine: a full and potent agonist at the hM4D(Gi) DREADD amenable to clinical translation of chemogenetics

    Weston et al (2018) bioRxiv doi: :
  • Microbiota imprint gut–intrinsic neuronal programming and sympathetic activity

    Muller and Mucida(2019) bioRxiv :
  • Pharmacokinetic and pharmacodynamic actions of clozapine-N-oxide, clozapine, and compound 21 in DREADD-based chemogenetics in mice.

    Jendryka et al (2019) Sci Rep. 9(1) : 4522
    PubMedID: 30872749
  • Olanzapine: A potent agonist at the hM4D(Gi) DREADD amenable to clinical translation of chemogenetics

    Weston M et al(2019) Sci Adv 5(4) : 1567
    PubMedID: 31001591
Support & Resources


  • Verified customer

    So far so good! We've been using compound 21 for about 3 months now to probe circuit specific effects in Cre rats. We have been able to detect some dose-dependent behavioral effects with a great degree of specificity. Compared to CNO, we feel that this DREADD drug is at least equivalent.
  • Verified customer

    Dissolves well, easy to use. Product easily dissolves in water, and injections seem to work as intended. Currently investigating whether oral consumption has similar effects in vivo.
  • Verified customer

    Excellent product, easy to dissolve in saline. In vivo results suggest effective inhibition of hM4D-expressing neurons (and accompanying behavioral phenotype) with 1mg/kg i.p. administration.
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