In my cart

You have no items in your shopping cart.

Compound 21 (DREADD agonist 21) dihydrochloride (water soluble)


Product overview

  • Name
    Compound 21 (DREADD agonist 21) dihydrochloride (water soluble)
  • Short description
    Highly potent hM3Dq DREADD agonist. Water soluble.
  • Biological description

    Compound 21 (DREADD agonist 21) hydrochloride is a water soluble analog of clozapine N-oxide (CNO). The Free base is also available.

    Compound 21 (DREADD agonist 21) is a highly potent agonist at the excitatory hM3Dq DREADD (EC50 = 1.7 nM) and shows high selectivity for activating hM3Dq compared to the native hM3 muscarinic receptor and other GPCRs.

    There are indications that Compound 21 cannot be metabolized via normal routes to clozapine (or any related compound), has minimal off-target activity and has equivalent potency in vivo when compared with clozapine N-oxide (CNO).

    Unpublished data indicates that Compound 21 (DREADD agonist 21) activates the inhibitory hM4D DREADD.

    Unpublished data also suggests using a dose of 3mg/kg in rat models.

    Data from a poster presented at SfN by the Michaelides group indicates that Compound 21 has lower binding affinity for DREADDs compared to clozapine and that Compound 21 induces behavioural effects in transgenic mice. 1mg/kg doses were used. High doses (10mg/kg) may cause sedation.

    Compound 21 therefore represents an alternative to CNO.

  • Alternative names
  • Purity
  • Citations



  • Chemical name
    11-(1-Piperazinyl)-5H-dibenzo[b,e][1,4]diazepine dihydrochloride
  • Molecular Weight
  • Chemical structure
    Compound 21 hydrochloride
  • Molecular Formula
  • Source
  • InChi
  • InChiKey
  • Appearance
    Yellow solid

Storing and Using Your Product

  • Storage instructions
  • Solubility overview
    Soluble in water (100mM). Always store solutions at -20°C.
  • Handling

    • Hydroscopic solid, contact with air may cause material to become sticky. Product performance should not be affected but we recommend storing the material in a sealed jar.
    • Always store solutions at -20°C.

  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

References for Compound 21 (DREADD agonist 21) dihydrochloride (water soluble)

  • The first structure-activity relationship studies for designer receptors exclusively activated by designer drugs.

    Chen et al (2015) ACS Chem Neurosci 6(3) : 476-84
    PubMedID: 25587888
  • Optogenetic approaches for dissecting neuromodulation and GPCR signaling in neural circuits.

    Spangler and Bruchas (2017) Curr Opin Pharmacol 32(4) : 56-70.
    PubMedID: 27875804
  • Clozapine N-Oxide Administration Produces Behavioral Effects in Long-Evans Rats: Implications for Designing DREADD Experiments.

    MacLaren et al (2016) eNeuro 3(5) : 0219-16
    PubMedID: 27822508
  • New non-CNO actuators for DREADDs

    Roth Bl (2015) Blog : N/A
Support & Resources