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Data

Product overview

  • Name
    UBP145
  • Short description
    Selective antagonist for GluN2D subunit containing NMDA receptors
  • Biological description

    Selective antagonist for GluN2D subunit containing NMDA receptors. Binds competitively at the glutamate site.

    Displays ~10- fold selectivity for GluN2D-containing receptors over GluN2B (NR2B) or GluN2A (NR2A) containing receptors (Ki values are 1.19, 7.99 and 11.53 at GluN1/GluN2D, GluN1/GluN2B and GluN1/GluN2A containing receptors respectively).

    Inhibits synaptic NMDAR response.

  • Alternative names
    UBP-145
  • Biological action
    Antagonist
  • Purity
    >98%
  • Citations

Properties

  • Chemical name
    1-(9-bromophenanthrene-3-carbonyl)piperazine-2,3-dicarboxylic acid
  • Molecular Weight
    457.28
  • Chemical structure
    UBP 145
  • Molecular Formula
    C21H17BrN2O5
  • PubChem identifier
    56945293
  • SMILES
    C1CN([C@H]([C@H](N1)C(=O)O)C(=O)O)C(=O)C2=CC3=C(C=C2)C=C(C4=CC=CC=C43)Br
  • Source
    Synthetic
  • InChi
    InChI=1S/C21H17BrN2O5/c22-16-10-11-5-6-12(9-15(11)13-3-1-2-4-14(13)16)19(25)24-8-7-23-17(20(26)27)18(24)21(28)29/h1-6,9-10,17-18,23H,7-8H2,(H,26,27)(H,28,29)/t17-,18+/m0/s1
  • InChiKey
    HUVFBBHRFWAJAA-ZWKOTPCHSA-N

Storing and Using Your Product

  • Storage instructions
    -20°C
  • Solubility overview
    Soluble in DMSO (100mM, gentle warming)
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for UBP145

  • N-methyl-D-aspartate (NMDA) receptor NR2 subunit selectivity of a series of novel piperazine-2,3-dicarboxylate derivatives: preferential blockade of extrasynaptic NMDA receptors in the rat hippocampal CA3-CA1 synapse.

    Costa et al (2009) J Pharmacol Exp Ther 331(2) : 618-26
    PubMedID: 19684252
  • GluN2B and GluN2D NMDARs dominate synaptic responses in the adult spinal cord.

    Hildebrand et al (2014) Sci Rep 13 : 4-4094
    PubMedID: 24522697
  • Different NMDA receptor subtypes mediate induction of long-term potentiation and two forms of short-term potentiation at CA1 synapses in rat hippocampus in vitro.

    Volianskis et al (2013) J Physiol. 591(4) : 955-72
    PubMedID: 23230236
Support & Resources

Product FAQ's

How do I solubilise (dissolve) D-AP5?

You can solubilise D-AP5 in water, up to a concentration of 100 mM. Once D-AP5 is in suspension, if you have problems solubilising it, you can try:

  • Stirring – try rapidly stirring or vortexing in a whirlimixer
  • Heating – try warming it gently in a water bath
  • Sonicating – sonication may also be worth a try   

Temperature is very important when solubilising biochemicals. For example if you’ve cooled or frozen your D-AP5 solution, it may have precipitated out of solution. So here’s an important rule – make sure you check that D-AP5 is fully re-dissolved before use.

Do I need to re-weigh the D-AP5 in my vial?

Yes - you should weigh out the quantity of D-AP5 that you require for your experiment as the amount of product in our vials isn’t weighed out accurately enough for direct addition of solution. There are some exceptions to this but if this is the case, it will be stated clearly on the datasheet.

How do I store D-AP5?

Storing D-AP5 as a solid: If you keep the vial kept tightly sealed at room temperature, you can store D-AP5 for up to 6months.

Storing and working with solutions of D-AP5:
If you are planning on storing and working with solutions of D-AP5, we recommend preparing and using your solutions on the same day. However, if this isn’t possible and you need to prepare stock solutions of D-AP5 beforehand, you should aliquot out the solution into tightly sealed vials at store at -20°C. We generally recommend that these will be useable for up to one month. You should also allow D-AP5 to equilibrate to RT for at least one hour before opening and using.

How do I make a 30 uM concentration of Cmpd101 in aCSF, from an initial stock made in 100% DMSO?

If you prepare a stock solution of Cmpd101 dissolved in 100% DMSO to a concentration of 10-30 mM, you should then be able to take the final concentration down to 30 uM in ACSF (final DMSO 0.1%).

How do I solubilise (dissolve) Cmpd101?

You can solubilise Cmpd101 in DMSO, up to a concentration of 100 mM.
Once Cmpd101 is in suspension, if you have problems solubilising it, you can try:

  • Stirring – try rapidly stirring or vortexing in a whirlimixer
  • Heating – try warming it gently in a water bath
  • Sonicating – sonication may also be worth a try   

Temperature is very important when solubilising biochemicals. For example if you’ve cooled or frozen your Cmpd101 solution, it may have precipitated out of solution. So here’s an important rule – make sure you check that Cmpd101 is fully re-dissolved before use.

Do I need to re-weigh the Cmpd101 in my vial?

Yes - you should weigh out the quantity of Cmpd101 that you require for your experiment as the amount of product in our vials isn’t weighed out accurately enough for direct addition of solution. There are some exceptions to this but if this is the case, it will be stated clearly on the datasheet.

How do I store Cmpd101?

Storing Cmpd101 as a solid: If you keep the vial kept tightly sealed at room temperature, you can store Cmpd101 for up to 6months.

Storing and working with solutions of Cmpd101:
If you are planning on storing and working with solutions of Cmpd101, we recommend preparing and using your solutions on the same day. However, if this isn’t possible and you need to prepare stock solutions of Cmpd101  beforehand, you should aliquot out the solution into tightly sealed vials at store at -20°C. We generally recommend that these will be useable for up to one month. You should also allow Cmpd101 to equilibrate to RT for at least one hour before opening and using.

What should CNO (freebase) look like?

CNO (freebase) is an amorphous, yellow powder

What temperature should I store the solid compound at?

We undertake regular quality control analysis of the solid compound and have found no deterioration in product purity when stored at room temperature. 

We recommend that you store solid CNO (freebase) powder at room temperature (desiccate).

Is the solid powder light sensitive?

No - we have found no evidence for Hello Bio CNO (free base) to be sensitive to light.

Is the solid powder air sensitive?

No - we have found no evidence for Hello Bio CNO (freebase) to be sensitive to air.

How do I weigh the compound to make my stock solution?

You should weigh out the quantity of product that you require for your experiment as the amount of product in our vials isn’t weighed out accurately enough for direct addition of solution.

How do I solubilize CNO to make my stock solution? Which solvent / buffer can I use?

Batch to batch variation in the solubility of CNO (freebase) can occur. Although some batches are soluble in aqueos soution, the ongoing solubility of these solutions can be unpredictable. This has been shown in the literature and in our stability studies.

Therefore, when working with CNO (freebase) we recommending using DMSO (100mM).

Various groups have different approaches to solubilizing CNO. DMSO is commonly used to dissolve CNO before addition to saline for injection.

What should I do if I find precipitate in my stock solution?

Gently heat your solution in a water bath to ~40°C and the compound should readily re-dissolve. Always take care to ensure that the compound is completely dissolved before use.

What concentration should my stock solution be?

This depends on your experiment and the working concentration you require.

What should my stock solution look like?

When dissolved your solution of CNO (freebase) should be a clear, yellow to orange color (depending on the concentration)

Can I store my stock solution of Clozapine N- oxide (CNO (freebase)?

We recommend making up solutions and using them immediately. Do not store solutions.

How long does Clozapine N-oxide (CNO freebase) stay stable in solution?

Our stability studies have shown that CNO in solution remains chemically stable (>99% purity by HPLC) for at least 4 weeks at room temperature. Please see stabiltiy data below.

Is my stock solution light sensitive?

No - we have found no evidence that Hello Bio CNO (freebase) is light sensitive.

Is my stock solution air sensitive?

No - we have found no evidence that Hello Bio CNO (freebase) is air sensitive.