Product overview

  • Name
    Kainic acid
  • Short description
    Prototypic, selective kainate receptor agonist
  • Biological description
    Prototypic, selective kainate receptor agonist. Potent excitant and neurotoxin. Widely used to model epilepsy and neurodegenerative diseases.
  • Biological action
    Agonist
  • Purity
    >98%
  • Customer comments

    We tested the Kainic acid and we are very happy with it. Hello Bio Kainic acid was used at 25 mg/kg i.p. and successfully induced status epilepticus. Verified customer, CNRS, France

  • Our products in action

Images

Properties

  • Chemical name
    (2S,3S,4S)-Carboxy-4-(1-methylethenyl)-3-pyrrolidineacetic acid
  • Molecular Weight
    213.23
  • Chemical structure
    Kainic acid  [487-79-6]
  • Molecular Formula
    C10H15NO4
  • CAS Number
    487-79-6
  • PubChem identifier
    10255
  • SMILES
    CC(=C)[C@H]1CNC([C@H]1CC(=O)O)C(=O)O
  • Source
    Extracted from diginea simplex
  • InChi
    InChI=1S/C10H15NO4/c1-5(2)7-4-11-9(10(14)15)6(7)3-8(12)13/h6-7,9,11H,1,3-4H2,2H3,(H,12,13)(H,14,15)/t6-,7+,9-/m0/s1
  • InChiKey
    VLSMHEGGTFMBBZ-OOZYFLPDSA-N
  • MDL number
    MFCD00150833
  • Appearance
    White solid

Applications

  • Application notes

    The kainate receptor (KAR) agonist kainic acid induces neuronal oscillations in the gamma frequency band (35-100Hz). KAR activation leads to synchronous activity of excitatory and inhibitory network firing. Kainic acid is commonly used at concentrations of 400-600 nM to induce oscillations in acute brain slices. At a concentration of 550 nM, kainic acid from Hello Bio induces a strong gamma frequency neuronal oscillation (approx. 35Hz) within the CA3 region of the rat hippocampus (see Fig 1 above). Oscillations were abolished following kainic acid washout.

     

    #Protocol 1: Oscillations assay

    • Acute rat hippocampal slices were placed in an interface chamber and held at 30°C with a glass recording electrode placed in the CA3 region of the hippocampus.
    • Control basal activity was recorded for 1 hr in the presence of aCSF before continuous perfusion of aCSF containing kainic acid at 550nM to induce oscillations.
    • Oscillation activity was recorded once oscillations had stabilised (~2-3 hours). 
    • Kainic acid was then washed out and replaced with control aCSF. 
    • Data were analysed to create a power spectrum of the oscillation.

     

Storing and Using Your Product

  • Storage instructions
    Room temperature
  • Solubility overview
    Soluble in water (25mM, gentle warming)

References for Kainic acid

  • Kainic acid-mediated excitotoxicity as a model for neurodegeneration.

    Wang Q et al (2005) Mol Neurobiol 31(1-3) : 3-16.
    PubMedID: 15953808
  • Distinct modulation of the endocannabinoid system upon kainic acid-induced in vivo seizures and in vitro epileptiform bursting.

    Fezza F et al (2014) Mol Cell Neurosci 62 : 1-9.
    PubMedID: 25064144
  • Identification and characterization of the ligand binding subunit of a kainic acid receptor using monoclonal antibodies and peptide mapping.

    Hampson DR et al (1989) J Biol Chem 264(22) : 13329-35.
    PubMedID: 2546953
  • Pharmacological characterization of glutamatergic agonists and antagonists at recombinant human homomeric and heteromeric kainate receptors in vitro.

    Alt et al (2004) Neuropharmacology 46(6) : 793-806
    PubMedID: 15033339

Reviews

  • Verified customer, CNRS, France

    We tested the Kainic acid and we are very happy with it. Hello Bio Kainic acid was used at 25 mg/kg i.p. and successfully induced status epilepticus.