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Product overview

  • Name
    A 83-01
  • Short description
    Selective TGF-βRI (ALK5), ALK4 and ALK7 inhibitor. Maintains human induced pluripotent stem cell (hiPSC) self renewal.
  • Biological description

    A 83-01 is a selective TGF-βRI (ALK5), ALK4 and ALK7 inhibitor (IC50 values are 12, 45 and 7.5 nM at ALK5, ALK4 and ALK7 respectively).


    A 83-01 aids reprogramming of fibroblasts into neural stem cells and cardiomyocytes.


    A 83-01 blocks phosphorylation of SMAD2/3 to inhibit TGF-β-induced epithelial-to-mesenchymal transition

    Maintenance / self-renewal

    A 83-01 helps to maintain homogeneity and long-term in vitro self-renewal of iPSCs

  • Alternative names
  • Biological action
  • Purity
  • Customer comments

    : Good quality and very affordable price. My lab are very happy with this product from Hello Bio, we will definitely purchase this product again. Verified customer, UK

  • Citations



  • Chemical name
    3-(6-Methyl-2-pyridinyl)-N-phenyl-4 -(4-quinolinyl)-1H-pyrazole-1-carbothioamide
  • Molecular Weight
  • Chemical structure
    A 83-01 |  [909910-43-6]
  • Molecular Formula
  • CAS Number
  • PubChem identifier
  • InChi
  • InChiKey
  • Appearance
    Pale yellow solid


  • Application notes

    Figures 1 - 3 show liver hepatocellular carcinoma tumouroids cultured with A 83-01 and forskolin

Storing and Using Your Product

  • Storage instructions
    -20°C (protect from light)
  • Solubility overview
    Soluble in DMSO (50mM)
  • Handling

    • This compound is light sensitive; we therefore recommend protecting the solid material and solutions from exposure to light.
    • It has been reported that A 83-01 can decompose to A 77-01 in solution. We therefore recommend that you make up solutions and use immediately. Do not store solutions.
    • If storage of solutions is required, you should aliquot out the solution into tightly sealed vials and store at -20°C and store these for up to one month.

  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for A 83-01

  • The ALK-5 inhibitor A-83-01 inhibits Smad signaling and epithelial-to-mesenchymal transition by transforming growth factor-beta.

    Tojo et al (2005) Cancer Sci 96(11) : 791-800
    PubMedID: 16271073
  • Brief report: combined chemical treatment enables Oct4-induced reprogramming from mouse embryonic fibroblasts.

    Yuan et al (2011) 2011 29(3) : 549-53
    PubMedID: 21425417
  • Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors.

    Li et al (2009) Cell Stem Cell 4(1) : 16-9
    PubMedID: 19097958
  • Chemical compound-based direct reprogramming for future clinical applications.

    Takeda et al (2018) Biosci Rep 38(3) : pii: BSR20171650
    PubMedID: 29739872
Support & Resources


  • Verified customer

    Good quality and very affordable price. My lab are very happy with this product from Hello Bio, we will definitely purchase this product again.