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Data

Product overview

  • Name
    A 83-01
  • Short description
    Selective TGF-βRI (ALK5), ALK4 and ALK7 inhibitor. Maintains human induced pluripotent stem cell (hiPSC) self renewal.
  • Biological description

    A 83-01 is a selective TGF-βRI (ALK5), ALK4 and ALK7 inhibitor (IC50 values are 12, 45 and 7.5 nM at ALK5, ALK4 and ALK7 respectively).

    Reprogramming

    A 83-01 aids reprogramming of fibroblasts into neural stem cells and cardiomyocytes.


    Differentiation

    A 83-01 blocks phosphorylation of SMAD2/3 to inhibit TGF-β-induced epithelial-to-mesenchymal transition


    Maintenance / self-renewal

    A 83-01 helps to maintain homogeneity and long-term in vitro self-renewal of iPSCs

  • Alternative names
    A83
  • Biological action
    Inhibitor
  • Purity
    >98%
  • Citations

Properties

  • Chemical name
    3-(6-Methyl-2-pyridinyl)-N-phenyl-4 -(4-quinolinyl)-1H-pyrazole-1-carbothioamide
  • Molecular Weight
    421.52
  • Chemical structure
    A 83-01 |  [909910-43-6]
  • Molecular Formula
    C25H19N5S
  • CAS Number
    909910-43-6
  • PubChem identifier
    16218924
  • SMILES
    CC1=CC=CC(=N1)C2=NN(C=C2C3=CC=NC4=CC=CC=C34)C(=S)NC5=CC=CC=C5
  • InChi
    InChI=1S/C25H19N5S/c1-17-8-7-13-23(27-17)24-21(19-14-15-26-22-12-6-5-11-20(19)22)16-30(29-24)25(31)28-18-9-3-2-4-10-18/h2-16H,1H3,(H,28,31)
  • InChiKey
    HIJMSZGHKQPPJS-UHFFFAOYSA-N
  • Appearance
    Pale yellow solid

Storing and Using Your Product

  • Storage instructions
    -20°C (protect from light)
  • Solubility overview
    Soluble in DMSO (50mM)
  • Handling

    • This compound is light sensitive; we therefore recommend protecting the solid material and solutions from exposure to light.
    • It has been reported that A 83-01 can decompose to A 77-01 in solution. We therefore recommend that you make up solutions and use immediately. Do not store solutions.
    • If storage of solutions is required, you should aliquot out the solution into tightly sealed vials and store at -20°C and store these for up to one month.

  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for A 83-01

  • The ALK-5 inhibitor A-83-01 inhibits Smad signaling and epithelial-to-mesenchymal transition by transforming growth factor-beta.

    Tojo et al (2005) Cancer Sci 96(11) : 791-800
    PubMedID: 16271073
  • Brief report: combined chemical treatment enables Oct4-induced reprogramming from mouse embryonic fibroblasts.

    Yuan et al (2011) 2011 29(3) : 549-53
    PubMedID: 21425417
  • Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors.

    Li et al (2009) Cell Stem Cell 4(1) : 16-9
    PubMedID: 19097958
  • Chemical compound-based direct reprogramming for future clinical applications.

    Takeda et al (2018) Biosci Rep 38(3) : pii: BSR20171650
    PubMedID: 29739872
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