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Data

Product overview

  • Name
    DL-AP5
  • Short description
    Competitive NMDA receptor antagonist
  • Biological description

    DL-AP5 is a competitive NMDA receptor antagonist which binds at the glutamate site.

    Impairs learning and fear conditioning.

    Water soluble DL-AP5 sodium salt also available.

  • Alternative names
    DL-APV
  • Biological action
    Antagonist
  • Purity
    >99%
  • Customer comments

    DL-AP5: can't get a better deal than this! We love Hello Bio's DL-AP5!! This is one of the most heavily used inhibitors in our electrophysiology lab and with three rigs running daily we go through a LOT of it... Verified customer, University of Toronto

  • Citations

Images

Properties

  • Chemical name
    DL-2-Amino-5-phosphonopentanoic acid
  • Molecular Weight
    197.13
  • Chemical structure
    DL-AP5  [76326-31-3]
  • Molecular Formula
    C5H12NO5P
  • CAS Number
    76326-31-3
  • PubChem identifier
    1216
  • SMILES
    NC(CCCP(=O)(O)O)C(=O)O
  • Source
    Synthetic
  • InChi
    InChI=1S/C5H12NO5P/c6-4(5(7)8)2-1-3-12(9,10)11/h4H,1-3,6H2,(H,7,8)(H2,9,10,11)
  • InChiKey
    VOROEQBFPPIACJ-UHFFFAOYSA-N
  • MDL number
    MFCD00010515
  • Appearance
    White solid

Applications

  • Application notes

    DL-AP5 from Hello Bio reduces the evoked NMDAR current at concentrations of 1 and 10 μM with full receptor antagonism achieved at 50 μM (see Fig 1 above). It was dissolved in water at 10 mM.

     

    #Protocol 1: Evoked NMDA receptor currents

    • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
    • NMDA currents were evoked via a stimulating electrode placed in layers II/III and evoked by a single square (150 μs) pulse every 10 sec at a stimulus intensity that gave a reliable NMDA current.
    • Neurons were held a +40 mV to relieve NMDA currents from their voltage-dependent Mg2+ block.
    • NMDA currents were continually stimulated and recorded in response to continual bath applications of DL-AP5 until NMDA currents were completely abolished.
    • All NMDAR recordings were made in the presence of GABAA-R and AMPAR antagonists.

Storing and Using Your Product

  • Storage instructions
    Room temperature
  • Solubility overview
    Soluble in water (10mM) or 0.1M NaOH (100mM)
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for DL-AP5

  • Infusion of the NMDA receptor antagonist, DL-APV, into the basolateral amygdala disrupts learning to fear a novel and a familiar context as well as relearning to fear an extinguished context.

    Laurent V et al (2009) Learn Mem 16(1) : 96-105.
    PubMedID: 19141468
  • The basolateral amygdala is necessary for learning but not relearning extinction of context conditioned fear.

    Laurent V et al (2008) Learn Mem 15(5) : 304-14.
    PubMedID: 18463174
  • Comparative analysis of different competitive antagonists interaction with NR2A and NR2B subunits of NMDA ionotropic glutamate receptor.

    Blaise MC et al (2005) J Mol Model 11(6) : 489-502.
    PubMedID: 15928921
  • Context-Dependent Modulation of Excitatory Synaptic Strength by Synaptically Released Zinc

    Kalappa and Tzounopoulos (2017) eNeuro 10.1523 : 0011-17
Our products in action
The following papers have cited the use of DL-AP5 (HB0251) from Hello Bio. If you have published a paper using this product, and it is not shown here, then please tell us! We will send you a free gift as a thank you!
  • Context-Dependent Modulation of Excitatory Synaptic Strength by Synaptically Released Zinc

    Kalappa and Tzounopoulos(2017) eNeuro 10.1523 : 0011-17
  • PAR1 activation induces rapid changes in glutamate uptake and astrocyte morphology.

    Sweeny et al (2017) Sci Rep. 7 : 43606
    PubMedID: 28256580
  • Mechanisms of synaptic zinc plasticity at mouse dorsal cochlear nucleus glutamatergic synapses

    Tzounopoulos and Vogler(2018) bioRxiv doi : https://doi.org/10.1101/320671
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Reviews

  • Verified customer, University of Toronto

    DL-AP5: can't get a better deal than this! We love Hello Bio's DL-AP5!! This is one of the most heavily used inhibitors in our electrophysiology lab and with three rigs running daily we go through a LOT of it. We switched from another supplier to Hello Bio's DL-AP5 two years ago and the 50% price difference has saved us thousands of dollars so far without sacrificing an iota of quality! All those savings are used on other aspects of our research program rather than lining the pockets of industry executives and I call that a win for the taxpayers that fund our research!