Product overview

  • Name
    DNQX
  • Short description
    Selective, competitive AMPA / kainate receptor antagonist
  • Biological description
    Selective and competitive AMPA and kainate receptor antagonist. Also acts as partial AMPA agonist in the presence of γ2 transmembrane AMPA receptor regulatory proteins (TARP) subunit. A neuroleptic agent that displays pro-oxidant activity.
  • Biological action
    Antagonist
  • Purity
    >98%
  • Our products in action

Images

Properties

  • Chemical name
    6,7-Dinitroquinoxaline-2,3-dione
  • Molecular Weight
    252.14
  • Chemical structure
    DNQX  [2379-57-9]
  • Molecular Formula
    C8H4N4O6
  • CAS Number
    2379-57-9
  • PubChem identifier
    3899541
  • SMILES
    C1=C2C(=CC(=C1[N+](=O)[O-])[N+](=O)[O-])NC(=O)C(=O)N2
  • Source
    Synthetic
  • InChi
    InChI=1S/C8H4N4O6/c13-7-8(14)10-4-2-6(12(17)18)5(11(15)16)1-3(4)9-7/h1-2H,(H,9,13)(H,10,14)
  • InChiKey
    RWVIMCIPOAXUDG-UHFFFAOYSA-N
  • MDL number
    MFCD00069257
  • Appearance
    Pale yellow solid

Applications

  • Application notes

    DNQX antagonizes the actions of glutamate at AMPA receptors. It is commonly used to reduce excitatory post synaptic currents (EPSC) and is commonly used at 10 μM. DNQX disodium salt from Hello Bio completely blocks both spontaneous and evoked EPSCs at 10 μM, with concentrations of 1 μM also effective (see Fig 1 above).

     

    #Protocol 1: Evoked and spontaneous excitatory post synaptic currents (EPSCs)

    • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
    •  EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
    • Neurons were held at -70 to -60 mV (the reversal potential of GABA currents). EPSCs were continuously stimulated and recorded in response to 5 min applications of varying concentrations of NBQX disodium salt until complete receptor inhibition.
    • Spontaneous EPSCs were recorded before and after addition of NBQX disodium salt by holding the neuron at -70 mV and recording for 10 sec.
    • Recordings for EPSCs were made in the absence of GABAA-R antagonists.

Storing and Using Your Product

  • Storage instructions
    Room temperature
  • Solubility overview
    Soluble in DMSO (100mM)
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for DNQX

  • Redox properties and prooxidant cytotoxicity of a neuroleptic agent 6,7-dinitrodihydroquinoxaline-2,3-dione (DNQX).

    Šarlauskas J et al (2013) Acta Biochim Pol 60(2) : 227-31.
    PubMedID: 23757451
  • TARP auxiliary subunits switch AMPA receptor antagonists into partial agonists.

    Menuz K et al (2007) Science 318(5851) : 815-7.
    PubMedID: 17975069
  • Selective excitatory actions of DNQX and CNQX in rat thalamic neurons.

    Lee SH et al (2010) J Neurophysiol 103(4) : 1728-34.
    PubMedID: 20107128
  • Pharmacological characterization of glutamatergic agonists and antagonists at recombinant human homomeric and heteromeric kainate receptors in vitro.

    Alt et al (2004) Neuropharmacology 46(6) : 793-806
    PubMedID: 15033339