NBQX disodium salt

(HB0443)

Product overview

  • Name
    NBQX disodium salt
  • Short description
    Potent, selective, competitive AMPA receptor antagonist. Disodium salt.
  • Biological description

    Potent, selective and competitive AMPA receptor antagonist. Also kainate receptor antagonist. Water soluble, disodium salt. Shows neuroprotective, antinociceptive and anticonvulsive actions. NBQX also available.

  • Biological action
    Antagonist
  • Purity
    >98%
  • Customer comments

    High quality and affordable! We use this compound routinely in the lab for neuronal recordings. Verified customer, The University of Montana

    Worked just as it should, results indistinguishable from our previous product but at a significant cost reduction! Verified customer, The University of Toronto

    Good quality and great price! Verified customer, The University of Newcastle

    NBQX disodium salt produced by Hello Bio produced a very potent and "clean" block of synaptic AMPA currents, with no effect on other GABAA or NMDA receptors.Verified customer, The University of Edinburgh

  • Our products in action

Images

Properties

  • Chemical name
    2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium salt
  • Molecular Weight
    380.24
  • Chemical structure
    NBQX disodium salt  [479347-86-9]
  • Molecular Formula
    C12H6N4Na2O6S
  • CAS Number
    479347-86-9
  • PubChem identifier
    3272523
  • SMILES
    [Na+].[Na+].NS(=O)(=O)c3cccc2c3c(cc1nc([O-])c([O-])nc12)[N+]([O-])=O
  • Source
    Synthetic
  • InChi
    InChI=1S/C12H8N4O6S.2Na/c13-23(21,22)8-3-1-2-5-9(8)7(16(19)20)4-6-10(5)15-12(18)11(17)14-6;;/h1-4H,(H,14,17)(H,15,18)(H2,13,21,22);;/q;2*+1/p-2
  • InChiKey
    SVJKYIUJRJEABK-UHFFFAOYSA-L
  • MDL number
    MFCD12910445
  • Appearance
    Orange solid

Applications

  • Application notes

    The AMPA receptor antagonist NBQX disodium salt inhibits the actions of glutamate by acting at AMPARs and is commonly used at 10 μM. NBQX disodium salt from Hello Bio inhibits spontaneous and evoked excitatory post synaptic currents (EPSCs) (see Fig 1 above). Complete AMPA receptor blockade was achieved at 10 μM and NBQX disodium salt was also effective at reducing these currents at 1 μM. 

     

    #Protocol 1: Evoked and spontaneous excitatory post synaptic currents (EPSCs)

    • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
    •  EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
    • Neurons were held at -70 to -60 mV (the reversal potential of GABA currents). EPSCs were continuously stimulated and recorded in response to 5 min applications of varying concentrations of NBQX disodium salt until complete receptor inhibition.
    • Spontaneous EPSCs were recorded before and after addition of NBQX disodium salt by holding the neuron at -70 mV and recording for 10 sec.
    • Recordings for EPSCs were made in the absence of GABAA-R antagonists.

Storing and Using Your Product

  • Storage instructions
    -20°C
  • Solubility overview
    Soluble in water (100mM)

References for NBQX disodium salt

  • It is AMPA receptor, not kainate receptor, that contributes to the NBQX-induced antinociception in the spinal cord of rats.

    Kong LL et al (2006) Brain Res 1100(1) : 73-7.
    PubMedID: 16777075
  • Competitive inhibition by NBQX of kainate/AMPA receptor currents and excitatory synaptic potentials: importance of 6-nitro substitution.

    Randle JC et al (1992) Eur J Pharmacol 215(2-3) : 237-44.
    PubMedID: 1382998
  • Both MK801 and NBQX reduce the neuronal damage after impact-acceleration brain injury.

    Goda M et al (2002) J Neurotrauma 19(11) : 1445-56.
    PubMedID: 12490009
  • Antiepileptogenic and anticonvulsant effects of NBQX, a selective AMPA receptor antagonist, in the rat kindling model of epilepsy.

    Namba T et al (1994) Brain Res 638(1-2) : 36-44.
    PubMedID: 8199874
  • Pharmacological characterization of glutamatergic agonists and antagonists at recombinant human homomeric and heteromeric kainate receptors in vitro.

    Alt et al (2004) Neuropharmacology 46(6) : 793-806
    PubMedID: 15033339
  • PAR1 activation induces rapid changes in glutamate uptake and astrocyte morphology.

    Sweeny et al (2017) Sci Rep. 7 : 43606
    PubMedID: 28256580
The following papers have cited the use of NBQX disodium salt (HB0443) from Hello Bio. If you have published a paper using this product, and it is not shown here, then please tell us! We will send you a free gift as a thank you!
  • mGluR-LTD at Excitatory and Inhibitory Synapses in the Lateral Habenula Tunes Neuronal Output.

    Valentinova and Mameli( 2016) Cell Rep 16(9) : 2298-307
    PubMedID: 27545888

Reviews

  • Verified customer, The University of Newcastle

    We use your NBQX in the lab regularly.
  • Verified customer, University of Bristol

    I use NBQX disodium from Hello Bio to block AMPARs/KARs and isolate NMDAR EPSPs.
  • Verified customer, the University of Edinburgh

    NBQX produced by Hello Bio produced a very potent and "clean" block of synaptic AMPA currents, with no effect on other GABAA or NMDA receptors.
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