Product overview

  • Name
    NBQX
  • Short description
    Potent, selective, competitive AMPA receptor antagonist
  • Biological description

    Potent, selective and competitive AMPA receptor antagonist. Also kainate receptor antagonist. Shows neuroprotective, antinociceptive and anticonvulsive actions. Water soluble, NBQX disodium salt also available.

  • Alternative names
    NBQX
  • Biological action
    Antagonist
  • Purity
    >98%
  • Our products in action

Images

Properties

  • Chemical name
    2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide
  • Molecular Weight
    336.28
  • Chemical structure
    NBQX  [118876-58-7]
  • Molecular Formula
    C12H8N4O6S
  • CAS Number
    118876-58-7
  • PubChem identifier
    3272524
  • SMILES
    C1=CC2=C3C(=CC(=C2C(=C1)S(=O)(=O)N)[N+](=O)[O-])NC(=O)C(=O)N3
  • Source
    Synthetic
  • InChi
    InChI=1S/C12H8N4O6S/c13-23(21,22)8-3-1-2-5-9(8)7(16(19)20)4-6-10(5)15-12(18)11(17)14-6/h1-4H,(H,14,17)(H,15,18)(H2,13,21,22)
  • InChiKey
    UQNAFPHGVPVTAL-UHFFFAOYSA-N
  • MDL number
    MFCD11046016
  • Appearance
    Yellow solid

Applications

  • Application notes

    The AMPA receptor antagonist NBQX inhibits the actions of glutamate acting at AMPARs and is commonly used at 10 μM. NBQX from Hello Bio reduces spontaneous and evoked excitatory post synaptic currents (EPSCs) (see Fig 1 above). Complete AMPA receptor blockade was achieved at 10 μM and NBQX was also effective at 1 μM. NBQX was dissolved in DMSO. 

     

    #Protocol 1: Evoked and spontaneous excitatory post synaptic currents (EPSCs)

    • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
    • EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
    • Neurons were held at -70 to -60 mV (the reversal potential of GABA currents). EPSCs were continuously stimulated and recorded in response to 5 min applications of varying concentrations of NBQX until complete receptor inhibition.
    • Spontaneous EPSCs were recorded before and after addition of NBQX by holding the neuron at -70 mV and recording for 10 sec.
    • Recordings for EPSCs were made in the absence of GABAA-R antagonists.

Storing and Using Your Product

  • Storage instructions
    Room temperature
  • Solubility overview
    Soluble in DMSO (100mM)
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for NBQX

  • It is AMPA receptor, not kainate receptor, that contributes to the NBQX-induced antinociception in the spinal cord of rats.

    Kong LL et al (2006) Brain Res 1100(1) : 73-7.
    PubMedID: 16777075
  • Competitive inhibition by NBQX of kainate/AMPA receptor currents and excitatory synaptic potentials: importance of 6-nitro substitution.

    Randle JC et al (1992) Eur J Pharmacol 215(2-3) : 237-44.
    PubMedID: 1382998
  • Both MK801 and NBQX reduce the neuronal damage after impact-acceleration brain injury.

    Goda M et al (2002) J Neurotrauma 19(11) : 1445-56.
    PubMedID: 12490009
  • Antiepileptogenic and anticonvulsant effects of NBQX, a selective AMPA receptor antagonist, in the rat kindling model of epilepsy.

    Namba T et al (1994) Brain Res 638(1-2) : 36-44.
    PubMedID: 8199874
  • Pharmacological characterization of glutamatergic agonists and antagonists at recombinant human homomeric and heteromeric kainate receptors in vitro.

    Alt et al (2004) Neuropharmacology 46(6) : 793-806
    PubMedID: 15033339

Reviews

  • Verified customer, University of Kiel

    It works as it should