D-AP5

(HB0225)

Product overview

  • Name
    D-AP5
  • Short description
    Selective, competitive NMDA receptor antagonist
  • Biological description

    Widely used, selective and competitive NMDA receptor antagonist which binds at glutamate site. More active form of DL-AP5. Blocks induction of LTP (long term potentiation) and impairs spatial learning.

  • Alternative names
     2-APV, D-APV
  • Biological action
    Antagonist
  • Purity
    >99%
  • Customer comments

    I made the discovery that the NMDA receptor is the trigger for the induction of LTP using D-AP5 synthesized by Jeff Watkins, the discoverer of the NMDA receptor... I now obtain my D-AP5 from Hello Bio. I love their products and ethos and that is why I accepted a position on their Scientific Advisory Board.

    Professor Graham Collingridge, winner of The Brain Prize, 2016

    My lab used D-AP5 from Hello Bio and were very happy with it. It behaved exactly as expected! Professor Kei Cho, Chair of Neuroscience, University of Bristol, UK (Hello Bio Scientific Advisory Board Member)

    My lab is very satisfied with your D-AP5 quality and price. Verified customer, European Brain Research Institute (EBRI)

  • Citations

Images

Properties

  • Chemical name
    D-(-)-2-Amino-5-phosphonopentanoic acid
  • Molecular Weight
    197.13
  • Chemical structure
    D-AP5 APV  [79055-68-8]
  • Molecular Formula
    C5H12NO5P
  • CAS Number
    79055-68-8
  • PubChem identifier
    135342
  • SMILES
    N[C@H](CCCP(=O)(O)O)C(=O)O
  • Source
    Synthetic
  • InChi
    InChI=1S/C5H12NO5P/c6-4(5(7)8)2-1-3-12(9,10)11/h4H,1-3,6H2,(H,7,8)(H2,9,10,11)/t4-/m1/s1
  • InChiKey
    VOROEQBFPPIACJ-SCSAIBSYSA-N
  • MDL number
    MFCD00078839
  • Appearance
    White solid

Applications

  • Application notes

    The NMDA receptor antagonist D-AP5 is commonly used to inhibit NMDA mediated synaptic plasticity. It is often used at concentrations of 50 μM. D-AP5 from Hello Bio completely abolishes evoked NMDAR mediated currents at 50 μM and reduces NMDA currents at lower concentrations of 1 and 10 μM (see Fig 1 above).

     

    #Protocol 1: Evoked NMDA receptor currents

    • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
    •  NMDA currents were evoked via a stimulating electrode placed in layers II/III and evoked by a single square (150 μs) pulse every 10 sec at a stimulus intensity that gave a reliable NMDA current.
    • Neurons were held a +40 mV to relieve NMDA currents from their voltage-dependent Mg2+ block.
    • NMDA currents were continually stimulated and recorded in response to continual bath applications of D-AP5 until NMDA currents were completely abolished.
    • All NMDAR recordings were made in the presence of GABAA-R and AMPAR antagonists.

     

Storing and Using Your Product

  • Storage instructions
    Room temperature
  • Solubility overview
    Soluble in water (100mM)
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for D-AP5

  • NMDA receptors, learning and memory: chronic intraventricular infusion of the NMDA receptor antagonist d-AP5 interacts directly with the neural mechanisms of spatial learning.

    Morris RG et al (2013) Eur J Neurosci 37(5) : 700-17.
    PubMedID: 23311352
  • Actions of D and L forms of 2-amino-5-phosphonovalerate and 2-amino-4-phosphonobutyrate in the cat spinal cord.

    Davies J et al (1982) Brain Res 235(2) : 378-86.
    PubMedID: 6145492
  • Effects of pre or posttraining dorsal hippocampus D-AP5 injection on fear conditioning to tone, background, and foreground context.

    Schenberg EE et al (2008) Hippocampus 18(11) : 1089-93.
    PubMedID: 18727044
The following papers have cited the use of D-AP5 (HB0225) from Hello Bio. If you have published a paper using this product, and it is not shown here, then please tell us! We will send you a free gift as a thank you!
  • Calcium-Permeable AMPA Receptors Mediate the Induction of the Protein Kinase A-Dependent Component of Long-Term Potentiation in the Hippocampus.

    Park et al(2016 ) J Neurosci 36(2) : 622-31
    PubMedID: 26758849
  • SALM5 trans-synaptically interacts with LAR-RPTPs in a splicing-dependent manner to regulate synapse development.

    Choi et al (2016) Sci Rep 26 : 6:26676
    PubMedID: 27225731
  • mGluR-LTD at Excitatory and Inhibitory Synapses in the Lateral Habenula Tunes Neuronal Output.

    Valentinova and Mameli(2016) Cell Rep 16(9) : 2298-307
    PubMedID: 27545888
  • Structural basis of subunit selectivity for competitive NMDA receptor antagonists with preference for GluN2A over GluN2B subunits.

    Lind et al(2017) Proc Natl Acad Sci U S A. doi: 10.1073/pnas.1707752114 : Epub ahead of print
    PubMedID: 28760974
  • NMDA receptors contribute to retrograde synaptic transmission from ganglion cell photoreceptors to dopaminergic amacrine cells

    Liu et al(2017) Frontier in Cellular Neuroscience 11:279 : (1-13)
  • Serotonin 2B receptors in mesoaccumbens dopamine pathway regulate cocaine responses

    Doly et al(2017) J Neurosci DOI: https://doi.org/10.1523/JNEUROSCI : 1354-17
    PubMedID: 28935766
  • Synaptic co-transmission of acetylcholine and GABA regulates hippocampal states

    Takacs et al(2017) bioRxiv 10. : 1101/193318
  • Limiting habenular hyperactivity ameliorates maternal separation-driven depressive-like symptoms.

    Tchenio et al(2017) Nat Commun 8(1) : 1135
    PubMedID: 29074844
  • Presynaptic mGluR5 receptor controls glutamatergic input through protein kinase C-NMDA receptors in paclitaxel-induced neuropathic pain.

    Xie et al(2017) J Biol Chem pii: jbc.M117.818476. : doi: 10.1074/jbc.M117.818476.
    PubMedID: 29074619

Product FAQ's

How do I solubilise (dissolve) D-AP5?

You can solubilise D-AP5 in water, up to a concentration of 100 mM. Once D-AP5 is in suspension, if you have problems solubilising it, you can try:

  • Stirring – try rapidly stirring or vortexing in a whirlimixer
  • Heating – try warming it gently in a water bath
  • Sonicating – sonication may also be worth a try   

Temperature is very important when solubilising biochemicals. For example if you’ve cooled or frozen your D-AP5 solution, it may have precipitated out of solution. So here’s an important rule – make sure you check that D-AP5 is fully re-dissolved before use.

Do I need to re-weigh the D-AP5 in my vial?

Yes - you should weigh out the quantity of D-AP5 that you require for your experiment as the amount of product in our vials isn’t weighed out accurately enough for direct addition of solution. There are some exceptions to this but if this is the case, it will be stated clearly on the datasheet.

How do I store D-AP5?

Storing D-AP5 as a solid: If you keep the vial kept tightly sealed at room temperature, you can store D-AP5 for up to 6months.

Storing and working with solutions of D-AP5:
If you are planning on storing and working with solutions of D-AP5, we recommend preparing and using your solutions on the same day. However, if this isn’t possible and you need to prepare stock solutions of D-AP5 beforehand, you should aliquot out the solution into tightly sealed vials at store at -20°C. We generally recommend that these will be useable for up to one month. You should also allow D-AP5 to equilibrate to RT for at least one hour before opening and using.

Reviews

  • Verified customer, The University of Newcastle

    We regularly use your D-AP5 in the lab.
  • Verified customer, University of Bristol

    I block NMDA receptors using APV from Hello Bio and am very happy with the quality.
  • Dr. Graham Collingridge, Lunenfeld-Tanenbaum

    I purchase my D-AP5 from Hello Bio a company that I would highly recommend - the results are indistinguishable from D-AP5 sourced from higher priced suppliers.
Page:
  1. 1
  2. 2