Hoechst 33342

(HB0787)

Product overview

  • Name
    Hoechst 33342
  • Short description
    Fluorescent DNA dye
  • Biological description
    Binds to minor grove of AT-rich regions of DNA. Fluorescence increases greatly upon DNA binding. Excited by ultraviolet light. Membrane permeable. Also used to stain fixed cells as DAPI substitute.
  • Biological action
    Dyes & stains
  • Our products in action

Properties

  • Chemical name
    Bisbenzimide
  • Molecular Weight
    561.93
  • Chemical structure
    Hoechst 33342  [23491-52-3]
  • Molecular Formula
    C27H28N6O.3HCl
  • CAS Number
    23491-52-3
  • PubChem identifier
    http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=1464
  • SMILES
    CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C

Storing and Using Your Product

  • Solubility overview
    soluble in water or DMSO
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for Hoechst 33342

  • Labeling nuclear DNA with hoechst 33342.

    Chazotte B (2011) Cold Spring Harb Protoc 2011(1) : pdb.prot5557.
    PubMedID: 21205857
  • Hoechst 33342 stain and u.v. laser exposure do not induce genotoxic effects in flow-sorted boar spermatozoa.

    Parrilla I et al (2004) Reproduction 128(5) : 615-21.
    PubMedID: 15509707
  • Phototoxicity of Hoechst 33342 in time-lapse fluorescence microscopy.

    Purschke M et al (2010) Photochem Photobiol Sci 9(12) : 1634-9.
    PubMedID: 20931137

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