(R,S)-AMPA

(HB0030)

Product overview

  • Name
    (R,S)-AMPA
  • Short description
    Prototypic AMPA receptor agonist
  • Biological description

    Prototypic AMPA receptor agonist (EC50 = 11 μM). (S)-AMPA is the active enantiomer form.

  • Biological action
    Agonist
  • Purity
    >98%
  • Citations

Images

Properties

  • Chemical name
    (RS)-α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid
  • Molecular Weight
    186.17
  • Chemical structure
    (R,S)-AMPA  [74341-63-2]
  • Molecular Formula
    C7H10N2O4
  • CAS Number
    74341-63-2
  • PubChem identifier
    1221
  • SMILES
    CC1=C(C(=O)NO1)CC(C(=O)O)N
  • Source
    Synthetic
  • InChi
    InChI=1S/C7H10N2O4/c1-3-4(6(10)9-13-3)2-5(8)7(11)12/h5H,2,8H2,1H3,(H,9,10)(H,11,12)
  • InChiKey
    UUDAMDVQRQNNHZ-UHFFFAOYSA-N
  • MDL number
    MFCD00213388
  • Appearance
    White solid

Applications

  • Application notes

    The AMPA receptor agonist (R,S)-AMPA is typically used at concentrations of 1-100 µM. At 10 µM, (R,S)-AMPA from Hello Bio induces a large depolarising current. This depolarising current was occluded in the presence of the AMPA receptor antagonist NBQX (20µM). (See Fig 1 above).


    #Protocol 1: (R,S)-AMPA protocol

    • Whole cell voltage clamp recordings of CA1 pyramidal neurons from the rat hippocampal brain slice.
    • Neurons were held at -60 mV and continuously perfused with aCSF in the presence of the GABA receptor antagonist gabazine (20µM).
    • AMPA currents were evoked via applying (R,S)-AMPA directly to the recording chamber during continuous perfusion.
    • To test the selectivity of (R,S)-AMPA to AMPA receptors, the experiment was repeated within the same neuron in the presence of the AMPA receptor antagonist NBQX (20 µM)
    • Under these conditions (R,S)-AMPA failed to induce a depolarising current.

Storing and Using Your Product

  • Storage instructions
    Room temperature
  • Solubility overview
    Soluble in water (10mM, gentle warming)
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for (R,S)-AMPA

  • The AMPA receptor binding site: focus on agonists and competitive antagonists.

    Stensbøl TB et al (2002) Curr Pharm Des 8(10) : 857-72.
    PubMedID: 11945136
  • Willardiines differentiate agonist binding sites for kainate- versus AMPA-preferring glutamate receptors in DRG and hippocampal neurons.

    Wong LA et al (1994) J Neurosci 14(6) : 3881-97.
    PubMedID: 7515954
  • Activation and desensitization of AMPA/kainate receptors by novel derivatives of willardiine.

    Patneau DK et al (1992) J Neurosci 12(2) : 595-606.
    PubMedID: 1371315