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Data

Product overview

  • Name
    RuBi-Glutamate
  • Short description
    RuBi - caged glutamate compound. Water soluble.
  • Biological description

    Ruthenium-bipyridine-trimethylphosphine (RuBi) - caged glutamate. Water soluble.


    RuBi-Glutamate can be excited by visible wavelengths and releases glutamate after one- or two- photon excitation. Glutamate release occurs in less than 50 ns.
    Displays fewer non-specific effects than MNI-caged-Glutamate.


    RuBi-Glutamate has a relatively high absorption cross section in the visible (blue) and has a high quantum efficiency of uncaging which allows use at low concentrations (e.g. lower concentrations than MNI-Glu). This partly avoids blockade of GABAergic transmission which occurs with other caged compounds.


    Two-photon uncaging of RuBi-Glutamate has a high spatial resolution and generates excitatory responses in individual dendritic spines with physiological kinetics. With laser beam multiplexing, two-photon RuBi-Glutamate uncaging can also be used to depolarize and fire pyramidal neurons with single-cell resolution.


    Therefore, RuBi-Glutamate enables photoactivation of neuronal dendrites and circuits with visible or two-photon light sources at single cell and single spine precision.


    When combined with RuBi-Glutamate uncaging, two-photon photostimulation can be applied to optically map inhibitory connections.

  • Alternative names
    RuBi-Glu, RuBIglutamate
  • Biological action
    Caged compound
  • Customer comments

    Hello Bio produce very high quality Rubi-Glutamate, which is capable of evoking single spine synaptic currents following 2-photon activation. The spatial and temporal dynamics of the uncaging are suitable for fast photolysis, mimicking accurately endogenous glutamate release. Verified customer, The University of Edinburgh

  • Citations

Images

Properties

  • Chemical name
    (bis(2,2'-Bipyridine-N,N')trimethylphosphine)-(S)-1-aminopropane-1,3-dicarboxylic acid ruthenium(2+) complex sodium hexafluorophosphate salt
  • Molecular Weight
    970.54
  • Chemical structure
    RuBi-Glutamate
  • Molecular Formula
    C28H32N5O4PRu.2NaPF6
  • PubChem identifier
    90488860
  • SMILES
    CP(C)C.C1=CC=NC(=C1)C2=CC=CC=N2.C1=CC=NC(=C1)C2=CC=CC=N2.C(CC(=O)[O-])C(C(=O)[O-])N.F[P-](F)(F)(F)(F)F.F[P-](F)(F)(F)(F)F.[Na+].[Na+].[Ru+2]
  • Source
    Synthetic
  • InChiKey
    SQQVSHWHIPVYSZ-UHFFFAOYSA-L
  • Appearance
    Orange solid

Applications

  • Application notes

    Uncaging of Rubi-Glutamate using a 0.5 µm spot of 780 nm light for 2 ms (blue line), 1 µm adjacent to dendritic spines (average of 8 spine shown) in a CA1 pyramidal cell from a 28 day old rat.

    Traces were acquired using a cesium gluconate based intracellular solution in voltage-clamp configuration, in the presence of bath applied 300 µM Rubi-Glutamate and 50 µM picrotoxin (also Hello Bio), producing small AMPA EPSCs at -70 mV (top) and large NMDA EPSCs at +40 mV (bottom).

Storing and Using Your Product

  • Storage instructions
    -20°C
  • Solubility overview
    Soluble in water (20mM)
  • Handling
    This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the material in the dark and protecting from light.
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for RuBi-Glutamate

  • RuBi-Glutamate: Two-Photon and Visible-Light Photoactivation of Neurons and Dendritic spines.

    Fino E et al (2009) Front Neural Circuits 3 : 2
    PubMedID: 19506708
  • A fast ruthenium polypyridine cage complex photoreleases glutamate with visible or IR light in one and two photon regimes.

    Salierno M et al (2010) J Inorg Biochem 104(4) : 418-22.
    PubMedID: 20060592
  • Dense, unspecific connectivity of neocortical parvalbumin-positive interneurons: a canonical microcircuit for inhibition?

    Packer AM et al (2011) J Neurosci 31(37) : 13260-71.
    PubMedID: 21917809
  • Dense inhibitory connectivity in neocortex.

    Fino and Yuste (2011) Neuron. 69(6) : 1188-203.
    PubMedID: 21435562
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Reviews

  • Verified customer, the University of Edinburgh

    Hello Bio produce very high quality Rubi-Glutamate, which is capable of evoking single spine synaptic currents following 2-photon activation. The spatial and temporal dynamics of the uncaging are suitable for fast photolysis, mimicking accurately endogenous glutamate release.