RuBi - caged glutamate compound. Water soluble.
Ruthenium-bipyridine-trimethylphosphine (RuBi) - caged glutamate. Water soluble.
RuBi-Glutamate can be excited by visible wavelengths and releases glutamate after one- or two- photon excitation. Glutamate release occurs in less than 50 ns.
Displays fewer non-specific effects than MNI-caged-Glutamate.
RuBi-Glutamate has a relatively high absorption cross section in the visible (blue) and has a high quantum efficiency of uncaging which allows use at low concentrations (e.g. lower concentrations than MNI-Glu). This partly avoids blockade of GABAergic transmission which occurs with other caged compounds.
Two-photon uncaging of RuBi-Glutamate has a high spatial resolution and generates excitatory responses in individual dendritic spines with physiological kinetics. With laser beam multiplexing, two-photon RuBi-Glutamate uncaging can also be used to depolarize and fire pyramidal neurons with single-cell resolution.
Therefore, RuBi-Glutamate enables photoactivation of neuronal dendrites and circuits with visible or two-photon light sources at single cell and single spine precision.
When combined with RuBi-Glutamate uncaging, two-photon photostimulation can be applied to optically map inhibitory connections.
Hello Bio produce very high quality Rubi-Glutamate, which is capable of evoking single spine synaptic currents following 2-photon activation. The spatial and temporal dynamics of the uncaging are suitable for fast photolysis, mimicking accurately endogenous glutamate release. Verified customer, The University of Edinburgh
(bis(2,2'-Bipyridine-N,N')trimethylphosphine)-(S)-1-aminopropane-1,3-dicarboxylic acid ruthenium(2+) complex sodium hexafluorophosphate salt
Uncaging of Rubi-Glutamate using a 0.5 µm spot of 780 nm light for 2 ms (blue line), 1 µm adjacent to dendritic spines (average of 8 spine shown) in a CA1 pyramidal cell from a 28 day old rat.
Traces were acquired using a cesium gluconate based intracellular solution in voltage-clamp configuration, in the presence of bath applied 300 µM Rubi-Glutamate and 50 µM picrotoxin (also Hello Bio), producing small AMPA EPSCs at -70 mV (top) and large NMDA EPSCs at +40 mV (bottom).
Storing and Using Your Product
Soluble in water (20mM)
This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the material in the dark and protecting from light.
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
References for RuBi-Glutamate
RuBi-Glutamate: Two-Photon and Visible-Light Photoactivation of Neurons and Dendritic spines.Fino E et al (2009) Front Neural Circuits 3 : 2PubMedID: 19506708
A fast ruthenium polypyridine cage complex photoreleases glutamate with visible or IR light in one and two photon regimes.Salierno M et al (2010) J Inorg Biochem 104(4) : 418-22.PubMedID: 20060592
Dense, unspecific connectivity of neocortical parvalbumin-positive interneurons: a canonical microcircuit for inhibition?Packer AM et al (2011) J Neurosci 31(37) : 13260-71.PubMedID: 21917809
Dense inhibitory connectivity in neocortex.Fino and Yuste (2011) Neuron. 69(6) : 1188-203.PubMedID: 21435562
Reviews & Product Guides
Hello Bio produce very high quality Rubi-Glutamate, which is capable of evoking single spine synaptic currents following 2-photon activation. The spatial and temporal dynamics of the uncaging are suitable for fast photolysis, mimicking accurately endogenous glutamate release.