Product overview

  • Name
    NMDA
  • Short description
    Prototypic NMDA receptor agonist
  • Biological description
    Prototypic NMDA receptor agonist which mimics the action of glutamate. Potent excitant.
  • Biological action
    Agonist
  • Purity
    >99%
  • Our products in action

Images

Properties

  • Chemical name
    N-Methyl-D-aspartic acid
  • Molecular Weight
    147.13
  • Chemical structure
    NMDA  [6384-92-5]
  • Molecular Formula
    C5H9NO4
  • CAS Number
    6384-92-5
  • PubChem identifier
    22880
  • SMILES
    CN[C@H](CC(=O)O)C(=O)O
  • InChi
    InChI=1S/C5H9NO4/c1-6-3(5(9)10)2-4(7)8/h3,6H,2H2,1H3,(H,7,8)(H,9,10)/t3-/m1/s1
  • InChiKey
    HOKKHZGPKSLGJE-GSVOUGTGSA-N
  • MDL number
    MFCD00004226

Applications

  • Application notes

    The prototypic NMDA receptor agonist NMDA is effective at a range of concentrations and typically used at 100 μM. NMDA from Hello Bio induces inward depolarising whole-cell currents in cortical neurons at 10 μM with prominent currents at 50 μM. The actions of NDMA were fully blocked by D-AP5 (NMDAR antagonist) at 100 μM (see Fig 1 above).

     

    #Protocol 1: NMDA mediated whole-cell currents

    • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
    • Neurons were held at -70 mV and continuously perfused with aCSF in the presence of AMPA and GABA receptor antagonists CNQX (10 μM) and Bicuculline (100 μM) respectively and Tetrodotoxin (1 μM) to reduce network activity.
    • NMDA currents were evoked by applying NMDA directly to the recording chamber during continuous perfusion.
      • To test the selectivity of NMDA to NMDA receptors the experiment was repeated within the same neuron in the presence of the NMDA receptor antagonist D-AP5 (100 μM). Under these conditions NMDA failed to induce a depolarising current.

Storing and Using Your Product

  • Storage instructions
    Room temperature
  • Solubility overview
    Soluble in water (100mM)
  • Important
    This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

References for NMDA

  • N-methyl-D-aspartic acid (NMDA) in the nervous system of the amphioxus Branchiostoma lanceolatum.

    D'Aniello S et al (2007) BMC Neurosci 8 : 109
    PubMedID: 18096065
  • Regulation of N-methyl-D-aspartic acid (NMDA) receptors by metabotropic glutamate receptor 7.

    Gu Z et al (2012) J Biol Chem 287(13) : 10265-75.
    PubMedID: 22287544
  • Occurrence of D-aspartic acid and N-methyl-D-aspartic acid in rat neuroendocrine tissues and their role in the modulation of luteinizing hormone and growth hormone release.

    D'Aniello A et al (2000) FASEB J 14(5) : 699-714.
    PubMedID: 10744627